J Vet Sci.  2017 Jun;18(2):237-243. 10.4142/jvs.2017.18.2.237.

Sequence analysis of the spike gene of Porcine epidemic diarrhea virus isolated from South China during 2011–2015

• Zhao X
• Li Z
• Zeng X
• Zhang G
• Niu J
• Sun B
• Ma J

• KMID: 2412577
• DOI: http://doi.org/10.4142/jvs.2017.18.2.237

Abstract

The spike gene of porcine epidemic diarrhea virus (PEDV) was sequenced from 55 South China field strains isolated from pigs with symptoms of diarrhea. The sequences were compared within the set of field strains as well as with reference strains available in GenBank. Within the 55 South China PEDV field strains, the deduced amino acid sequence identities ranged from 93.8% to 99.9 % and ranged from 90.7% to 99.5% when compared with the foreign reference strains in GenBank. Our phylogenetic analysis showed that 10 of the 55 South China PEDV strains belonged to G1b and 45 belonged to G2b.

Keyword

Porcine epidemic diarrhea virus; molecular characterization; phylogenetic analysis

MeSH Terms

Amino Acid Sequence/genetics
Animals
China/epidemiology
Coronavirus Infections/*veterinary/virology
Genes, Viral/*genetics
Glycosylation
Phylogeny
Porcine epidemic diarrhea virus/*genetics
Sequence Alignment/veterinary
Swine
Swine Diseases/*microbiology

Figure

• 

  Fig. 1 Alignment of the deduced amino acid sequences of partial spike (S) protein of Guangdong isolates with that of vaccine strains. The dots represent amino acids that are identical to those in the CV777 strain. Boxes indicate the neutralizing epitopes (SS2, 748–755; SS6, 764–771; and 2C10, 1368–1374).

• 

  Fig. 2 Phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) strains based on nucleotide sequences of the complete spike glycoprotein S gene. The tree was constructed using the neighbor-joining method in MEGA 5.05 software, using 1,000 bootstrap replicates. Bootstrap values > 75% are shown at the branch points.

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