Exp Mol Med.  2001 Jun;33(2):76-82.

Cloning and characterization of 5'-upstream region of human phospholipase C-beta2 gene

• Yun ES
• Lee SJ
• Kim MJ
• Ryu SH
• Suh PG

Abstract

5'-upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/ -13) were identified in the upstream region of PLC-beta2 gene. We suggest that the transcription of PLC-beta2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.

Keyword

phospholipase C-b2; promoter; cloning; transcription

MeSH Terms

Aspergillus Nuclease S1/metabolism
Base Sequence
Cells, Cultured
Chloramphenicol O-Acetyltransferase/metabolism
Cloning, Molecular
Conserved Sequence
Gene Deletion
Isoenzymes/*chemistry/*genetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Phospholipase C/*chemistry/*genetics
Promoter Regions (Genetics)
Protein Binding
Support, Non-U.S. Gov't
Transcription, Genetic
Transfection