J Korean Pediatr Soc.  1999 Jun;42(6):833-843.

Molecular Cloning and Characterization of ETV1 : a New Member of the ETS Family Transcription Factor that is Implicated in Ewing's sarcoma

• Jeon IS
• Shapiro D

Abstract

PURPOSE: Ewing's sarcoma is characterized by accompanying specific chromosome translocations. In the present study, we report ETV1, the third member of the human ETS family that is implicated in Ewing's sarcoma by reciprocal translocation (7;22)(p22;q12).
METHODS
Primarily, cDNA library made from the Ewing's sarcoma cell line accompanying the t(7;22)(p22;q12) was screened using the 5' portion of EWS(including the exon 1-7) probe on the presumption that 5' portion of the fusion gene is made of EWS and 3' is a new member of the ETS family gene. After we obtained a 3' portion of ETV1, a member of the ETS family gene, an additional 5' portion was isolated by screening a human fetal brain cDNA library, using a 326 bp NcoI fragment derived from 3' portion of coding and uncoding sequences of this gene.
RESULTS
We cloned the whole sequence of ETV1. Based on extensive homology, ETV1 could be subdivided into human PEA3 sub-family with ERM and E1A-F.
CONCLUSION
The cloning of new oncogenes has begun to provide insight into the molecular pathogenesis of tumor formation. In this regard, the cloning of ETV1 implicated in Ewing's sarcoma might provide a clue to understand the formation of this tumor.

Keyword

Ewing's tumor; ETS gene family; ETV1

MeSH Terms

Brain
Cell Line
Clinical Coding
Clone Cells
Cloning, Molecular*
Cloning, Organism
Exons
Gene Library
Humans
Mass Screening
Oncogenes
Sarcoma, Ewing*
Transcription Factors*
Transcription Factors