J Korean Acad Nurs.  2010 Aug;40(4):580-588. 10.4040/jkan.2010.40.4.580.

Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease

• Kim Y
• Choe MA

• KMID: 1073080
• DOI: http://doi.org/10.4040/jkan.2010.40.4.580

Abstract

PURPOSE
The purpose of this study was to examine effects of decreased locomotor activity on mass, Type I and II fiber cross-sectional areas of ipsilateral and contralateral hindlimb muscles 21 days after establishing the Parkinson's disease rat model.
METHODS
The rat model was established by direct injection of 6-hydroxydopamine (6-OHDA, 50 microgram) into the left substantia nigra after stereotaxic surgery. Adult male Sprague-Dawley rats were assigned to one of two groups; the Parkinson's disease group (PD; n=17) and a sham group (S; n=8). Locomotor activity was assessed before and 21 days after the experiment. At 22 days after establishing the rat model, all rats were anesthetized and soleus and plantaris muscles were dissected from both ipsilateral and contralateral sides. The brain was dissected to identify dopaminergic neuronal death of substantia nigra in the PD group.
RESULTS
The PD group at 21 days after establishing the Parkinson's disease rat model showed significant decrease in locomotor activity compared with the S group. Weights and Type I and II fiber cross-sectional areas of the contralateral soleus muscle of the PD group were significantly lower than those of the S group.
CONCLUSION
Contralateral soleus muscle atrophy occurs 21 days after establishing the Parkinson's disease rat model.

Keyword

Parkinson's disease; Rats; Hindlimb; Muscle atrophy

MeSH Terms

Animals
Body Weight
Disease Models, Animal
Hindlimb
Male
*Motor Activity
Muscle Fibers, Skeletal/physiology
Muscle, Skeletal/*physiopathology
Nutritional Status
Parkinson Disease/*physiopathology
Rats
Rats, Sprague-Dawley

Figure

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  Figure 1 Experimental design.

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  Figure 2 TH-specific immunohistochemical staining in substantia nigra in unilateral 6-OHDA lesioned rats.

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