Exp Mol Med.  2008 Dec;40(6):639-646. 10.3858/emm.2008.40.6.639.

Transcriptional induction of DLC-1 gene through Sp1 sites by histone deacetylase inhibitors in gastric cancer cells

• Kim TY
• Kim IS
• Jong HS
• Lee JW
• Kim TY
• Jung M
• Bang YJ

• KMID: 1072944
• DOI: http://doi.org/10.3858/emm.2008.40.6.639

Abstract

We previously reported that trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, induced DLC-1 mRNA expression and accumulated acetylated histones H3 and H4 associated with the DLC-1 promoter in DLC-1 non-expressing gastric cancer cells. In this study, we demonstrated the molecular mechanisms by which TSA induced the DLC-1 gene expression. Treatment of the gastric cancer cells with TSA activates the DLC-1 promoter activity through Sp1 sites located at -219 and -174 relative to the transcription start site. Electrophoretic mobility-shift assay (EMSA) revealed that Sp1 and Sp3 specifically interact with these Sp1 sites and showed that TSA did not change their binding activities. The ectopic expression of Sp1, but not Sp3, enhances the DLC-1 promoter responsiveness by TSA. Furthermore, the TSA-induced DLC-1 promoter activity was increased by p300 expression and reduced by knockdown of p300. These results demonstrated the requirement of specific Sp1 sites and dependence of Sp1 and p300 for TSA-mediated activation of DLC-1 promoter.

Keyword

DLC1 protein, human; histone deacetylases; p300-CBP transcription factors; promoter regions, genetic; Sp1 transcription factor; trichostation A

MeSH Terms

Cell Line, Tumor
Electrophoretic Mobility Shift Assay
Histone Deacetylases/*antagonists & inhibitors/metabolism
Humans
Hydroxamic Acids/*pharmacology
Promoter Regions, Genetic
Sp1 Transcription Factor/genetics/*metabolism
Sp3 Transcription Factor/genetics/metabolism
Stomach Neoplasms/*metabolism
Transcription, Genetic
Tumor Suppressor Proteins/*biosynthesis/genetics
p300-CBP Transcription Factors/genetics/metabolism