Exp Mol Med.
2008 Dec;40(6):583-595. 10.3858/emm.2008.40.6.583.
Polarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus
- Affiliations
-
- 1Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju, Korea. vetvirus@chonbuk.ac.kr
- 2Department of Chemistry, Seonam University, Namwon, Korea.
- 3Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University, Jeonju, Korea.
- 4Department of Pharmacology, School of Medicine, Pusan National University, Busan, Korea.
- KMID: 1072939
- DOI: http://doi.org/10.3858/emm.2008.40.6.583
Abstract
- Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protective immunity against a lethal challenge. Among three constructs, recombinant adenovirus expressing gB (rAd-gB) was found to induce the most potent immunity biased to Th1-type, as determined by the IgG isotype ratio and the profile of the Th1/Th2 cytokine production. Conversely, the gC-expressing adenovirus (rAd-gC) revealed Th2-type immunity and the gD-expressing adenovirus (rAd-gD) induced lower levels of IFN-gamma and IL-4 production than other constructs, except IL-2 production. Mucosal delivery of rAd-gB induced mucosal IgA and serum IgG responses and biased toward Th2-type immune responses. However, these effects were not observed in response to systemic delivery of rAd-gB. In addition, rAd-gB appeared to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route. These results suggest that administration of replication-incompetent adenoviruses can induce different types of immunity depending on the expressed antigen and that recombinant adenoviruses expressing gB induced the most potent Th1-biased humoral and cellular immunity and provided effective protection against PrV infection.
MeSH Terms
-
Adenoviridae/genetics/*immunology/metabolism
Animals
Antibody Formation
Cell Line
Cytokines/immunology
Female
Glycoproteins/biosynthesis/genetics/*immunology
Herpesvirus 1, Suid/genetics/*immunology/physiology
Immunity, Cellular
Immunoglobulin G/immunology
Mice
Mice, Inbred C57BL
Pseudorabies/*immunology/prevention & control
Pseudorabies Vaccines/administration & dosage/*immunology
Swine
Th1 Cells/immunology
Th2 Cells/immunology
*Virus Replication
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