J Korean Med Sci.  2001 Feb;16(1):103-107. 10.3346/jkms.2001.16.1.103.

Age-related regional difference of interleukin-1 expression in rat brain after lipopolysaccharide treatment

• Huh GY
• Roh MS
• Bae HR

• KMID: 1066502
• DOI: http://doi.org/10.3346/jkms.2001.16.1.103

Abstract

Aging is associated with altered immune responses including dysregulation of cytokine production. Of cytokines, interleukin-1 (IL-1) family has been primarily involved with central nervous system. To evaluate the age-related different response of IL-1 family following peripheral administration of lipopolysaccharide (LPS), immunohistochemical study of IL-1beta and IL-1 receptor expression was performed on Sprague-Dawley rat brain. Experimental animals were divided into four groups; saline-treated young (3-5 months) and old (over 24 months), and LPS-treated young and old groups. After intraperitoneal (i.p.) injection of LPS, three to five rats within each group were killed at 1, 2, 4, 8 and 16 hr. After fixation in 4% neutral buffered formalin, the brain slices were paraffin-embedded. Immunohistochemical staining using labelled streptavidin biotin was performed. The results showed that IL-1beta immunoreactivity was seen in the endothelial cell of pons in both LPS-reated young and old rats, with slightly longer persistency in old group. IL-1RI immunoreactivity appeared initially in the neurons of cerebral cortex in LPS-treated old group, compared with predominantly the cerebellum in LPS-treated young group. In conclusion, our study shows that there is age-related, different neuronal localization of IL-1RI expression at different points of time after LPS treatment.

Keyword

Interleukin-1; Immunohistochemistry; Lipopolysaccharides; Aging; Rats; Brain

MeSH Terms

Age Factors
Animal
Brain Chemistry/drug effects*
Gene Expression Regulation/drug effects
Immunohistochemistry
Interleukin-1/genetics
Interleukin-1/analysis*
Lipopolysaccharides/toxicity*
Male
Rats
Rats, Sprague-Dawley
Receptors, Interleukin-1/analysis