Exp Mol Med.  2009 Jul;41(7):508-516. 10.3858/emm.2009.41.7.056.

Sodium tanshinone IIA sulfonate attenuates angiotensin II-induced collagen type I expression in cardiac fibroblasts in vitro

• Yang L
• Zou XJ
• Gao X
• Chen H
• Luo JL
• Wang ZH
• Liang QS
• Yang GT

• KMID: 1058025
• DOI: http://doi.org/10.3858/emm.2009.41.7.056

Abstract

Cardiac fibrosis occurs after pathological stimuli to the cardiovascular system. One of the most important factors that contribute to cardiac fibrosis is angiotensin II (Ang II). Accumulating studies have suggested that reactive oxygen species (ROS) plays an important role in cardiac fibrosis and sodium tanshinone IIA sulfonate (STS) possesses antioxidant action. We therefore examined whether STS depresses Ang II-induced collagen type I expression in cardiac fibroblasts. In this study, Ang II significantly enhanced collagen type I expression and collagen synthesis. Meanwhile, Ang II depressed matrix metalloproteinase-1 (MMP-1) expression and activity. These responses were attenuated by STS. Furthermore, STS depressed the intracellular generation of ROS, NADPH oxidase activity and subunit p47(phox) expression. In addition, N-acetylcysteine the ROS scavenger, depressed effects of Ang II in a manner similar to STS. In conclusion, the current studies demonstrate that anti-fibrotic effects of STS are mediated by interfering with the modulation of ROS.

Keyword

angiotensin II; collagen; fibrosis; heart; reactive oxygen species; tanshinone

MeSH Terms

Acetylcysteine/pharmacology
Angiotensin II/*antagonists & inhibitors/pharmacology
Animals
Blotting, Western
Cells, Cultured
Collagen Type I/*metabolism
Drugs, Chinese Herbal/*pharmacology
Fibroblasts/*drug effects/metabolism
Free Radical Scavengers/pharmacology
Matrix Metalloproteinase 1/metabolism
Myocardium/*cytology
NADPH Oxidase/metabolism
Oxidative Stress/drug effects
Phenanthrenes/*pharmacology
Rats
Rats, Wistar
Reactive Oxygen Species/metabolism