Exp Mol Med.  2009 Oct;41(10):728-736. 10.3858/emm.2009.41.10.079.

The adaptor protein LAD/TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein

• Park E
• Choi Y
• Ahn E
• Park I
• Yun Y

• KMID: 1009394
• DOI: http://doi.org/10.3858/emm.2009.41.10.079

Abstract

The adaptor protein, LAD/TSAd, plays essential roles in T cell activation. To further understand the functions of this protein, we performed yeast two-hybrid screening using TSAd as bait and identified 67 kDa laminin binding protein (LBP) as the interacting partner. Subsequently, TSAd-LBP interaction was confirmed in D1.1 T cell line. Upon costimulation by T cell receptor (TCR) plus laminin crosslinking or TCR plus integrin alpha6 crosslinking, LBP was coimmunoprecipitated with TSAd. Moreover, TCR plus laminin costimulation-dependent T cell migration was enhanced in D1.1 T cells overexpressing TSAd but was disrupted in D1.1 cells overexpressing dominant negative form of TSAd or TSAd shRNA. These data show that, upon TCR plus integrin costimulation, TSAd associates with LBP and mediates T lymphocyte migration.

Keyword

cell movement; focal adhesion protein-tyrosine kinases; integrins; laminin binding protein 67-kDa, rat; Sh2d2a protein, mouse

MeSH Terms

Adaptor Proteins, Signal Transducing/genetics/*metabolism
Amino Acids, Diamino/metabolism
Carrier Proteins/*metabolism
*Cell Movement
Humans
Integrin alpha6/metabolism
Jurkat Cells
Mutation
Protein Binding
RNA, Small Interfering/genetics
*Receptor Cross-Talk
Receptors, Antigen, T-Cell/metabolism
T-Lymphocytes/*metabolism
Transgenes
Two-Hybrid System Techniques