Korean J Immunol.  1998 Mar;20(1):75-80.

Identification of a Major Cytoplasmic Laminin Binding Protein as the Heat Shock Cognate Protein 70 in Highly Metastatic Human Colon Carcinoma Cells

• Woo HJ

Abstract

"It is not clear yet how the laminin is made in cytoplasm and is secreted by cells, though the significance of the interaction between laminin and cells during the development, cell differentiation, tumor growth, and metastasis is well known. Furthermore, it has been reported that aberrant expression. and secretion of laminin in malignant tumor cells, In this study I found a major cytoplasmic laminin binding protein (LBP), which was revealed as the heat shock cognate protein 70 (hsc70), in human colon carcinoma cells by cell surface labeling with '""I, laminin and gelatin affinity chromatography, peptide microsequencing, and immunoblot experiments. This suggests another function of hsc70 as the cytoplasmic LBP, which may play an important role in biosynthesis, assembly, and secretion of laminin in tumor cells."

Keyword

Hsc70; Laminin binding protein; Colon carcinoma

MeSH Terms

Carrier Proteins*
Cell Differentiation
Chromatography, Affinity
Colon*
Cytoplasm*
Gelatin
Hot Temperature*
Humans*
Laminin*
Neoplasm Metastasis
Shock*
Carrier Proteins
Gelatin
Laminin