- Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
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Joseph S, Thomas N, Thangapandian E, Singh VP, Verma R, Srivastava SK
- KMID: 1365009
- J Vet Sci.
- 2012 Mar;13(1):99-101.
- doi: 10.4142/jvs.2012.13.1.99
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared... -
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- Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays
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Choi IS, Yoo HS
- KMID: 1095123
- J Vet Sci.
- 2002 Sep;3(3):207-212.
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2... -
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- Synthesis of recombinant blood coagulation factor VIII (FVIII) heavy and light chains and reconstitution of active form of FVIII
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Oh SH, Lee MY, Song DW
- KMID: 1029938
- Exp Mol Med.
- 1999 Jun;31(2):95-100.
FVIII is synthesized as a single chain precursor of approximately 280 kD with the domain structure of A1-A2-B-A3-C1-C2 and it circulates as a series of metal ion-linked heterodimers that result from cleavages... -
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- Expression and localization of human papillomavirus type 16 E6 and E7 open reading frame proteins in human epidermal keratinocyte
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Kim KH, Yoon DJ, Moon YA, Kim YS
- KMID: 1381212
- Yonsei Med J.
- 1994 Mar;35(1):1-9.
- doi: 10.3349/ymj.1994.35.1.1
Over 60 different types of human papillomavirus (HPV) have been identified, and they are classified into high and low risk groups based on the risk for malignant progression of HPV... -
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- Reconstitution of class I MHC molecules expressed in E. coli and complexed with single antigenic peptides
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Kim J, Kim MY, Chwae YJ, Park JH
- KMID: 1081047
- J Korean Med Sci.
- 1997 Aug;12(4):280-285.
- doi: 10.3346/jkms.1997.12.4.280
The HLA-Cw3 heavy chain has been expressed at high level as insoluble protein aggregates in E. coli. The protein aggregates dissolved in strong denaturant solution were efficiently reconstituted by... -
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- Improvement of the Diagnostic Sensitivity of Scrub Typhus Using a Mixture of Recombinant Antigens Derived from Orientia tsutsugamushi Serotypes
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Kim YJ, Yeo SJ, Park SJ, Woo YJ, Kim MW, Kim SH, Chang IA, Jeon SH, Park BJ, Song GJ, Lee MG, Kim IS, Kim YW
- KMID: 1777553
- J Korean Med Sci.
- 2013 May;28(5):672-679.
- doi: 10.3346/jkms.2013.28.5.672
Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub... -
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- Allergenic Characterization of 27-kDa Glycoprotein, a Novel Heat Stable Allergen, from the Pupa of Silkworm, Bombyx mori
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Jeong KY, Son M, Lee JY, Park KH, Lee JH, Park JW
- KMID: 2359987
- J Korean Med Sci.
- 2016 Jan;31(1):18-24.
- doi: 10.3346/jkms.2016.31.1.18
Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase,... -
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- Development of Enzyme-Linked Immunosorbent Assays Using 2 Truncated ORF2 Proteins for Detection of IgG Antibodies Against Hepatitis E Virus
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Taherkhani R, Makvandi M, Farshadpour F
- KMID: 1791904
- Ann Lab Med.
- 2014 Mar;34(2):118-126.
- doi: 10.3343/alm.2014.34.2.118
BACKGROUND: Without appropriate culture systems for hepatitis E virus (HEV), sufficient natural viral proteins are difficult to generate for use in serological tests. Therefore, it is important to produce large... -
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- Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection
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Lee HW, Moon SU, Ryu HS, Kim YJ, Cho SH, Chung GT, Lin K, Na BK, Kong Y, Chung KS, Kim TS
- KMID: 1091836
- Korean J Parasitol.
- 2006 Mar;44(1):49-54.
- doi: 10.3347/kjp.2006.44.1.49
In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion... -
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- Effects of Antioxidant on Reduction of Hindlimb Muscle Atrophy Induced by Cisplatin in Rats
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Kim JI, Choe MA
- KMID: 1791866
- J Korean Acad Nurs.
- 2014 Aug;44(4):371-380.
- doi: 10.4040/jkan.2014.44.4.371
PURPOSE: The purpose of this study was to examine the effects of Cu/Zn SOD on reduction of hindlimb muscular atrophy induced by cisplatin in rats. METHODS: Forty-two rats were assigned to... -
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- Intracellular trafficking and metabolic turnover of yeast prepro-alpha-factor-SRIF precursors in GH3 cells
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Lee MA, Cheong KH, Shields D, Park SD, Hong SH
- KMID: 931879
- Exp Mol Med.
- 2002 Sep;34(4):285-293.
Chimeric genes coding for prepro region of yeast alpha-factor and anglerfish SRIF were expressed in rat GH3 cells to determine whether yeast signals could regulate hormone processing in mammalian... -
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- The Effectiveness of Recombinant Human Thyroid-Stimulating Hormone versus Thyroid Hormone Withdrawal Prior to Radioiodine Remnant Ablation in Thyroid Cancer: A Meta-Analysis of Randomized Controlled Trials
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Pak K, Cheon GJ, Kang KW, Kim SJ, Kim IJ, Kim EE, Lee DS, Chung JK
- KMID: 1796944
- J Korean Med Sci.
- 2014 Jun;29(6):811-817.
- doi: 10.3346/jkms.2014.29.6.811
We evaluated the efficacy of recombinant human thyroid-stimulating hormone (rhTSH) versus thyroid hormone withdrawal (THW) prior to radioiodine remnant ablation (RRA) in thyroid cancer. A systematic search of MEDLINE, EMBASE,... -
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- Expression and characterization of a mutant recombinant blood coagulation factor VIII (rFVIII (m)).
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Oh SH, Cho SY, Jin HS, Jeong DL, Jung SG, Oh KH
- KMID: 1380231
- Exp Mol Med.
- 2002 Jul;34(3):233-238.
In an earlier study, a site directed mutant rFVIII (rFVIII(m), Arg(336) -> Gln(336)) expressed in baculovirus-insect cell (Sf9) system was found to sustain high level activity during incubation at 37... -
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- Changes in Anti-Group A Rotavirus Antibody Seroprevalence and Levels in the Western Gyeongnam Province of Korea Over 16 Years
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Seo JH, Park JJ, Lim JY, Jun JS, Park CH, Woo HO, Youn HS, Kwon YC, Kang HL, Baik SC, Lee WK, Cho MJ, Rhee KH, Kim W
- KMID: 2158001
- J Korean Med Sci.
- 2013 Jan;28(1):55-61.
- doi: 10.3346/jkms.2013.28.1.55
To observe how anti-group A rotavirus antibody seropositivity rates and levels have changed in the western region of Gyeongnam Province, 2,030 serum samples collected at four collection periods (1989-1990, 1994-1995,... -
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- Intrathecal Gabapentin Increases Interleukin-10 Expression and Inhibits Pro-Inflammatory Cytokine in a Rat Model of Neuropathic Pain
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Lee BS, Jun IG, Kim SH, Park JY
- KMID: 1429210
- J Korean Med Sci.
- 2013 Feb;28(2):308-314.
- doi: 10.3346/jkms.2013.28.2.308
We examined the possible anti-inflammatory mechanisms of gabapentin in the attenuation of neuropathic pain and the interaction between the anti-allodynic effects of gabapentin and interleukin-10 (IL-10) expression in a rat... -
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- Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani
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Choi JH, Lee JH, Yu HS, Jeong HJ, Kim J, Hong YC, Kong HH, Chung DI
- KMID: 1720517
- Korean J Parasitol.
- 2006 Sep;44(3):187-196.
- doi: 10.3347/kjp.2006.44.3.187
The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and... -
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- Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression
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Sun J, Wang Y, Yang J, Du D, Li Z, Wei J, Yang A
- KMID: 1401759
- Exp Mol Med.
- 2012 Nov;44(11):674-683.
Relative deficiency in production of glycoprotein hormone erythropoietin (Epo) is a major cause of renal anemia. This study planned to investigate whether the hypoxia-regulated system of Epo expression, constructed by... -
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