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Properties of GST-CALM expressed in E. coli

Kim JA, Kim SR, Jung YK, Woo SY, Seoh JY, Hong YS, Kim HL

  • KMID: 1109309
  • Exp Mol Med.
  • 2000 Jun;32(2):93-99.
Clathrin-coated vesicles (CCVs) are involved in protein and lipid trafficking between intracellular compartments in eukaryotic cells. CCVs are composed of clathrin and assembly proteins. The clathrin assembly protein lymphoid...
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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

Jia LJ, Zhang SF, Qian NC, Xuan XN, Yu LZ, Zhang XM, Liu MM

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens...
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Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)

Kim JY, Lee MH, Jung KI, Na HY, Cha HS, Ko EM, Kim TJ

  • KMID: 1109321
  • Exp Mol Med.
  • 2003 Aug;35(4):310-316.
We have used a surface plasmon resonance biosensor (SPR, BIACORE 2000) to detect antibodies against glucose 6-phosphate isomerase (GPI) in synovial fluids of rheumatoid arthritis (RA) and osteoarthritis (OA). Recombinant...
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Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

Lv L, Li X, Liu G, Li R, Liu Q, Shen H, Wang W, Xue C, Cao Y

Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the...
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Distribution of Adenoviral Vector in Brain after Intravenous Administration

Jin JY, Moon CI, Moon CI, Kang WS, Jeong DC

The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed;...
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Transduction of yeast cytosine deaminase mediated by HIV-1 Tat basic domain into tumor cells induces chemosensitivity to 5-fluorocytosine

Lee H, Ryu J, Kim KA, Lee KS, Lee JY, Park JB, Park J, Choi SY

  • KMID: 755664
  • Exp Mol Med.
  • 2004 Feb;36(1):43-51.
Enzyme/prodrug approach is one of the actively developing areas for cancer therapy. In an effort to develop more effective enzyme/prodrug systems, cell-permeable cytosine deaminase was produced by fusing yeast cytosine...
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Identification of a putative transactivation domain in human Nanog

Oh JH, Do HJ, Yang HM, Moon SY, Cha KY, Chung HM, Kim JH

  • KMID: 1056238
  • Exp Mol Med.
  • 2005 Jun;37(3):250-254.
Nanog is a newly identified divergent homeodomain protein that directs the infinite propagation and sustains the pluripotency of embryonic stem cells. It has been reported that murine Nanog has two...
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Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells

Ahn HJ, Kim S, Nam HW

Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar...
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Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors

Kang SG, Lee EK, Schaus S, Henderson

  • KMID: 938883
  • Exp Mol Med.
  • 2001 Sep;33(3):174-178.
Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection,...
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Vaccination against Murine Toxoplasmosis Using Recombinant Toxoplasma gondii SAG3 Antigen Alone or in Combination with Quil A

Lee YH, Shin DW, lee JH, Nam HW, Ahn MH

PURPOSE: Surface antigen 3 (SAG3) of Toxoplasma gondii is very similar in structure to the major surface antigen 1 (SAG1). Although numerous studies have supported the importance of SAG1 in...
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Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen

Kim SH, Kim MO, Lee SR, Kim KS, Lee TH, Lee HT, Ha JH, Kim TY, Ryoo ZY

  • KMID: 1094144
  • Exp Mol Med.
  • 2006 Jun;38(3):196-202.
We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3'-flanking region exhibit brain tumors and lymphoma. In this study,...
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FKBP-12 Exhibits an Inhibitory Activity on Calcium Oxalate Crystal Growth in Vitro

Han IS, Nakagawa Y, Park JW, Suh MH, Suh SI, Shin SW, Ahn SY, Choe BK

Urolithiasis and calcium oxalate crystal deposition diseases are still significant medical problems. In the course of nephrocalcin cDNA cloning, we have identified FKBP-12 as an inhibitory molecule of calcium oxalate...
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Interaction between chicken protein tyrosine phosphatase 1 (CPTP1)-like rat protein phosphatase 1 (PTP1) and p60v-src in v-src-transformed Rat-1 fibroblasts

Jung EJ, Kim CW

  • KMID: 1121102
  • Exp Mol Med.
  • 2002 Dec;34(6):476-480.
CPTP1 is a nontransmembrane chicken protein tyrosine phosphatase having 92% sequence homology to the corresponding 321 amino acids of human protein tyrosine phosphatase 1B (HPTP1B). Using anti-CPTP1 antibody, we...
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TACI:Fc scavenging B cell activating factor (BAFF) alleviates ovalbumin-induced bronchial asthma in mice

Moon EY, Ryu SK

  • KMID: 1047085
  • Exp Mol Med.
  • 2007 Jun;39(3):343-352.
Asthma was induced by the sensitization and challenge with ovalbumin (OVA) in mice. B-cell activating factor (BAFF) plays a role in mature B cell generation and maintenance. Here, we investigated...
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