Exp Mol Med.  2002 Dec;34(6):476-480.

Interaction between chicken protein tyrosine phosphatase 1 (CPTP1)-like rat protein phosphatase 1 (PTP1) and p60v-src in v-src-transformed Rat-1 fibroblasts

• Jung EJ
• Kim CW

Abstract

CPTP1 is a nontransmembrane chicken protein tyrosine phosphatase having 92% sequence homology to the corresponding 321 amino acids of human protein tyrosine phosphatase 1B (HPTP1B). Using anti-CPTP1 antibody, we identified CPTP1-like rat PTP1 of 51 kappa Da in Rat-1 and v-src-transformed Rat-1 fibroblasts. Here we show that CPTP1-like rat PTP1 binds to p60v-src in vivo and CPTP1 also can associate with p60v-src in cell lysate of v-src- transformed Rat-1 fibroblasts. Interaction between HPTP1B-type PTPs, CPTP1-like rat PTP1 and CPTP1, and p60v-src was reduced by vanadate treatment for 13 h due to down regulation of the protein level of p60v-src in vivo. Interestingly, CPTP1-like rat PTP1 was coimmunoprecipitated with a 70-kappa Da protein which has a possibility to be tyrosine- phosphorylated by p60v-src in v-src-transformed Rat- 1 fibroblasts. These results suggest that HPTP1B- type PTPs may play an important role in p60src dependent signal pathway in eucaryotic cells.

Keyword

oncogene protein pp60 (v-src); phosphoprotein phosphatase; protein interaction mapping; protein-tyrosine-phosphatase

MeSH Terms

Animals
Blotting, Western
Cell Line, Transformed
Chickens
Female
Fibroblasts
Oncogene Protein pp60(v-src)/*metabolism
Phosphoprotein Phosphatase/genetics/*metabolism
Precipitin Tests
Protein Binding
Protein-Tyrosine-Phosphatase/genetics/*metabolism
Rabbits
Rats
Recombinant Fusion Proteins/genetics/metabolism