- In vitro development of canine somatic cell nuclear transfer embryos in different culture media
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Kim DH, No JG, Choi MK, Yeom DH, Kim DK, Yang BC, Yoo JG, Kim MK, Kim HT
- KMID: 2164534
- J Vet Sci.
- 2015 Jun;16(2):233-235.
- doi: 10.4142/jvs.2015.16.2.233
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos... -
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- Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
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Jang G, Hong SG, Lee BC
- KMID: 1067341
- J Vet Sci.
- 2011 Mar;12(1):83-89.
- doi: 10.4142/jvs.2011.12.1.83
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated... -
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- Relationship between pregnancy rate and serum progesterone concentration in cases of porcine embryo transfer
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Moon J, Choi JY, Kang JT, Park SJ, Kim SJ, Jang G, Lee BC
- KMID: 1737624
- J Vet Sci.
- 2014 Mar;15(1):167-171.
- doi: 10.4142/jvs.2014.15.1.167
The level of P4 at the time of embryo transfer (ET) is important. P4 concentrations and numbers of corpora lutea for 126 recipients were evaluated. Nuclear transfer embryos were transferred... -
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- Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos
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Song K, Lee E
- KMID: 1104240
- J Vet Sci.
- 2007 Mar;8(1):81-87.
- doi: 10.4142/jvs.2007.8.1.81
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell... -
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- Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
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Hwang IS, Bae HK, Cheong HT
- KMID: 1705552
- J Vet Sci.
- 2013 Sep;14(3):235-240.
- doi: 10.4142/jvs.2013.14.3.235
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor... -
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- Expression of polo-like kinase 1 in pre-implantation stage murine somatic cell nuclear transfer embryos
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Moon J, Roh S
- KMID: 2434771
- J Vet Sci.
- 2019 Jan;20(1):2-9.
- doi: 10.4142/jvs.2019.20.1.2
Somatic cell nuclear transfer (SCNT) has various applications in research, as well as in the medical field and animal husbandry. However, the efficiency of SCNT is low and the accurate... -
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- Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal
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Lee E, Song K
- KMID: 1106223
- J Vet Sci.
- 2007 Dec;8(4):415-421.
- doi: 10.4142/jvs.2007.8.4.415
The objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer (SCNT) blastocysts using oocytes and donor cells from slaughtered... -
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- A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
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Park SK, Roh S, Park JI
- KMID: 1737612
- J Vet Sci.
- 2014 Mar;15(1):73-80.
- doi: 10.4142/jvs.2014.15.1.73
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression... -
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- Post-mortem re-cloning of a transgenic red fluorescent protein dog
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Hong SG, Koo OJ, Oh HJ, Park JE, Kim M, Kim GA, Park EJ, Jang G, Lee BC
- KMID: 1365026
- J Vet Sci.
- 2011 Dec;12(4):405-407.
- doi: 10.4142/jvs.2011.12.4.405
Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this... -
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- Intrapancreatic ectopic splenic tissue found in a cloned miniature pig
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Koo OJ, Ha SK, Park SJ, Park HJ, Kim SJ, Kwon D, Kang JT, Moon JH, Park EJ, Jang G, Lee BC
- KMID: 2164536
- J Vet Sci.
- 2015 Jun;16(2):241-244.
- doi: 10.4142/jvs.2015.16.2.241
Somatic cell nuclear transfer (SCNT) is a cost-effective technique for producing transgenic pigs. However, abnormalities in the cloned pigs might prevent use these animals for clinical applications or disease modeling.... -
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- Microtubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type
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Kwon DJ, Lee YM, Hwang IS, Park CK, Yang BK, Cheong HT
- KMID: 1110854
- J Vet Sci.
- 2010 Jun;11(2):93-101.
- doi: 10.4142/jvs.2010.11.2.93
This study was conducted to evaluate the microtubule distribution following control of nuclear remodeling by treatment of bovine somatic cell nuclear transfer (SCNT) embryos with caffeine or roscovitine. Bovine somatic... -
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- Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
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Lee J, Park JI, Yun JI, Lee Y, Yong H, Lee ST, Park CK, Hyun SH, Lee GS, Lee E
- KMID: 2344311
- J Vet Sci.
- 2015 Sep;16(3):373-380.
- doi: 10.4142/jvs.2015.16.3.373
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear... -
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- Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes
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Lee E, Bhuiyan MM, Watanabe H, Matsuoka K, Fujise Y, Ishikawa H, Fukui Y
- KMID: 1726899
- J Vet Sci.
- 2009 Dec;10(4):285-292.
- doi: 10.4142/jvs.2009.10.4.285
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their... -
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- Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos
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Ahn GJ, Lee BC, Hwang WS
- KMID: 1103521
- J Vet Sci.
- 2001 Aug;2(2):131-137.
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows:... -
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- Reproductive ability of a cloned male detector dog and behavioral traits of its offspring
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Lee JH, Kim GA, Kim RS, Lee JS, Oh HJ, Kim MJ, Hong DK, Lee BC
- KMID: 2413142
- J Vet Sci.
- 2016 Sep;17(3):407-411.
- doi: 10.4142/jvs.2016.17.3.407
In 2007, seven detector dogs were produced by somatic cell nuclear transfer using one nuclear donor dog, then trained and certified as excellent detector dogs, similar to their donor. In... -
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- Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos
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Cho J, Kang S, Lee BC
- KMID: 1784643
- J Vet Sci.
- 2014 Jun;15(2):225-231.
- doi: 10.4142/jvs.2014.15.2.225
This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell... -
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- Production of transgenic pigs using a pGFAP-CreER(T2)/EGFP(LoxP) inducible system for central nervous system disease models
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Hwang SU, Eun K, Yoon JD, Kim H, Hyun SH
- KMID: 2412134
- J Vet Sci.
- 2018 May;19(3):434-445.
- doi: 10.4142/jvs.2018.19.3.434
Transgenic (TG) pigs are important in biomedical research and are used in disease modeling, pharmaceutical toxicity testing, and regenerative medicine. In this study, we constructed two vector systems by using... -
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- Production of α1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology
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Kang JT, Kwon DK, Park AR, Lee EJ, Yun YJ, Ji DY, Lee K, Park KW
- KMID: 2363339
- J Vet Sci.
- 2016 Mar;17(1):89-96.
- doi: 10.4142/jvs.2016.17.1.89
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease... -
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- SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer
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Kang JT, Kim HJ, Oh HJ, Hong SG, Park JE, Kim MJ, Kim MK, Jang G, Kim DY, Lee BC
- KMID: 1376198
- J Vet Sci.
- 2012 Jun;13(2):211-213.
- doi: 10.4142/jvs.2012.13.2.211
Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. In this study,... -
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- Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
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He XY, Ma LB, He XN, Si WT, Zheng YM
- KMID: 2413163
- J Vet Sci.
- 2016 Jun;17(2):145-152.
- doi: 10.4142/jvs.2016.17.2.145
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target... -
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