J Vet Sci.  2015 Jun;16(2):233-235. 10.4142/jvs.2015.16.2.233.

In vitro development of canine somatic cell nuclear transfer embryos in different culture media

• Kim DH
• No JG
• Choi MK
• Yeom DH
• Kim DK
• Yang BC
• Yoo JG
• Kim MK
• Kim HT

• KMID: 2164534
• DOI: http://doi.org/10.4142/jvs.2015.16.2.233

Abstract

The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.

Keyword

canine; G1/G2 media; in vitro culture; somatic cell nuclear transfer embryo

MeSH Terms

Animals
Blastocyst/cytology
Cloning, Organism/*veterinary
Culture Media/metabolism
Dogs/*embryology
Embryo Culture Techniques/*veterinary
*Embryonic Development
Nuclear Transfer Techniques/*veterinary
Culture Media

Figure

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  Fig. 1 Canine somatic cell nuclear transfer (SCNT) embryos cultured in G1/G2 sequential media. (A) Blastocyst-stage embryo after 7 days of culturing. (B) Canine SCNT blastocyst stained with DAPI.

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