- Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
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Hwang IS, Bae HK, Cheong HT
- KMID: 1705552
- J Vet Sci.
- 2013 Sep;14(3):235-240.
- doi: 10.4142/jvs.2013.14.3.235
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor... -
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- Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
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Jang G, Hong SG, Lee BC
- KMID: 1067341
- J Vet Sci.
- 2011 Mar;12(1):83-89.
- doi: 10.4142/jvs.2011.12.1.83
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated... -
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- In vitro development of canine somatic cell nuclear transfer embryos in different culture media
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Kim DH, No JG, Choi MK, Yeom DH, Kim DK, Yang BC, Yoo JG, Kim MK, Kim HT
- KMID: 2164534
- J Vet Sci.
- 2015 Jun;16(2):233-235.
- doi: 10.4142/jvs.2015.16.2.233
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos... -
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- Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos
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Song K, Lee E
- KMID: 1104240
- J Vet Sci.
- 2007 Mar;8(1):81-87.
- doi: 10.4142/jvs.2007.8.1.81
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell... -
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- Intrapancreatic ectopic splenic tissue found in a cloned miniature pig
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Koo OJ, Ha SK, Park SJ, Park HJ, Kim SJ, Kwon D, Kang JT, Moon JH, Park EJ, Jang G, Lee BC
- KMID: 2164536
- J Vet Sci.
- 2015 Jun;16(2):241-244.
- doi: 10.4142/jvs.2015.16.2.241
Somatic cell nuclear transfer (SCNT) is a cost-effective technique for producing transgenic pigs. However, abnormalities in the cloned pigs might prevent use these animals for clinical applications or disease modeling.... -
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- Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal
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Lee E, Song K
- KMID: 1106223
- J Vet Sci.
- 2007 Dec;8(4):415-421.
- doi: 10.4142/jvs.2007.8.4.415
The objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer (SCNT) blastocysts using oocytes and donor cells from slaughtered... -
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- Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes
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Lee E, Bhuiyan MM, Watanabe H, Matsuoka K, Fujise Y, Ishikawa H, Fukui Y
- KMID: 1726899
- J Vet Sci.
- 2009 Dec;10(4):285-292.
- doi: 10.4142/jvs.2009.10.4.285
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their... -
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- Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
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Lee J, Park JI, Yun JI, Lee Y, Yong H, Lee ST, Park CK, Hyun SH, Lee GS, Lee E
- KMID: 2344311
- J Vet Sci.
- 2015 Sep;16(3):373-380.
- doi: 10.4142/jvs.2015.16.3.373
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear... -
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- SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer
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Kang JT, Kim HJ, Oh HJ, Hong SG, Park JE, Kim MJ, Kim MK, Jang G, Kim DY, Lee BC
- KMID: 1376198
- J Vet Sci.
- 2012 Jun;13(2):211-213.
- doi: 10.4142/jvs.2012.13.2.211
Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. In this study,... -
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- Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
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He XY, Ma LB, He XN, Si WT, Zheng YM
- KMID: 2413163
- J Vet Sci.
- 2016 Jun;17(2):145-152.
- doi: 10.4142/jvs.2016.17.2.145
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target... -
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