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The Effects of Physical States of Phospholipids on the Incorporation and CB Binding Activity of Band 4.5 Protein of human Erythrocyte Membrane in Reconstituted Vesicles

Hah JS

Proteoliposomes were prepared by reconstitution of a solubilized band 4.5 protein of human erythrocyte membrane with a mixture of phosphatidylcholine(PC) and phosphatidylethanolamine(PE) of varying ratios. The incorporation of the band...
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Dual action of d-tubocurarine on large-conductance Ca2+-activated K+ channels from rat brain reconstituted into planar lipid bilayer

Chung S, Shin JH

  • KMID: 2071534
  • Korean J Physiol Pharmacol.
  • 1998 Oct;2(5):549-553.
Using the planar lipid bilayer method, we investigated the effect of d-tubocurarine (dTC) on the extracellular side of large-conductance Ca2+-activated K+ channel from rat brain. When the initial open probability (Po) of the...
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Water-Impermeable Occlusion Effect to Intercorneocyte Lipid Layers in Hairless Mice

Jiang S, Youl S, Lee SH

BACKGROUND: Stratum corneum lipids are arranged as intercellular membrane bilayers presumed to mediate the epidermal permeability barrier. Acute disruption in barrier function will initiate epidermal lipid synthesis, which can be...
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Development and Application of Cell-penetrating Peptides

Yi H

Intracellular transduction of hydrophilic macromolecules has been problematic owing to the biochemical restriction imposed by lipid bilayer of the cytoplasmic membrane. Several technologies have been developed to improve the intracellular...
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Mechanisms of Selective Antimicrobial Activity of Gaegurin 4

Kim H, Lee BJ, Lee MH, Hong SG, Ryu PD

Gaegurin 4 (GGN4), an antimicrobial peptide isolated from a Korean frog, is five times more potent against Gram-positive than Gram-negative bacteria, but has little hemolytic activity. To understand the mechanism...
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Differences in the Recovery Rate after Perturbation of Epidermal Barrier by Means of Acetone Treatment and Tape-Stripping Technique

Chung HS, Lee SH

BACKGROUND: The epidermal permeability barrier necessary for terrestrial life resides in the intercellular spaces of the stratum corneum and is composed of lipids. OBJECTIVE: Since strrtum corneum lipid may be important...
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Barrier Rcovery after Topically Applied Desoxymethasone Ontment, Vaseline and Hydrobase on Benzalkonium Chloride-irritated Hairless Mice Skin

Ahn SK, Choi EH, Shaojun J, Hwang SM, Lee SH

  • KMID: 2303423
  • Korean J Dermatol.
  • 1998 Oct;36(5):820-826.
BACKGROUND: Topical irritants disrupt the cutaneous permeability barrier through the removal of stratum comeum lipids. This perturbation of barrier integrity stimulates a variety of homeostatic repair responses that ultimately result...
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Increased activity of large conductance Ca2+-activated K+ channels in negatively-charged lipid membranes

Park JB, Ryu PD

  • KMID: 2285216
  • Korean J Physiol Pharmacol.
  • 1998 Aug;2(4):529-539.
The effects of membrane surface charge originated from lipid head groups on ion channels were tested by analyzing the activity of single large conductance Ca2+-activated K+ (maxi K) channel from rat skeletal muscle....
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The Effect of Methanol on the Structural Parameters of Neuronal Membrane Lipid Bilayers

Joo HJ, Ahn SH, Lee HR, Jung SW, Choi CW, Kim MS, Bae MK, Chung IK, Bae SK, Jang HO, Yun I

The structures of the intact synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortexs, and the outer and the inner monolayer separately, were evaluated with 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane...
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The Effect of Lidocaine.HCl on the Fluidity of Native and Model Membrane Lipid Bilayers

Park JS, Jung TS, Noh YH, Kim WS, Park WI, Kim YS, Chung IK, Sohn UD, Bae SK, Bae MK, Jang HO, Yun I

The purpose of this study is to investigated the mechanism of pharmacological action of local anesthetic and provide the basic information about the development of new effective local anesthetics. Fluorescent...
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Epidermal Barrier in Human Fetus and Newborn

Lee SM, Lee EH, Jun JS, Kim DK, Lee SH

  • KMID: 2250266
  • Korean J Dermatol.
  • 2003 Jan;41(1):65-77.
BACKGROUND: The lipids of the stratum corneum, which originate from polar lipid precursors provided by the cells of the stratum granulosum via the exocytosis of lamellar bodies, with cornified cell...
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Decreasing Effect of Lidocaine.HCl on the Thickness of the Neuronal and Model Membrane

Park SM, Park JS, Kim JH, Baek JH, Yoon TG, Lee DK, Ryu WH, Chung IK, Sohn UD, Jang HO, Yun I

This study examined the mechanism of action of a local anesthetic, lidocaine.HCl. Energy transfer between the surface fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid, and the hydrophobic fluorescent probe, 1,3-di(1-pyrenyl) propane, was used...
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The Effect of Solvents Polarity on the Skin Barrier in Hairless Mice

Choi TJ, Yoon DH, Kim HO, Kim CW

  • KMID: 2085947
  • Korean J Dermatol.
  • 1997 Aug;35(4):660-666.
BACKGROUND: The stratom corneum lipids, responsible for the epidermal water bar rier, consist mainly of ceramides, cholesterol and free fatty acids. However, little has been studied about the effects of...
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The Effect of Barrier Recovery using Topical Steroid and Systemic Steroid after Acetone-application in Hairless Mice

Ahn SK, Chun SH, Choi EH, Lee SH

  • KMID: 2086585
  • Korean J Dermatol.
  • 2002 Oct;40(10):1173-1180.
BACKGROUND: Acetone disrupts the cutaneous permeability barrier through the removal of stratum corneum lipids. This pertubation of barrier integrity stimulates a variety of homeostatic repair that ultimately results in the...
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The Changes in the Skin Permeability Barrier and Epidermais of Hairless Mouse on Oleic Acid Treatment

Choi EH, Ahn SK, Lee SH

  • KMID: 2085952
  • Korean J Dermatol.
  • 1997 Aug;35(4):702-711.
BACKGROUND: The stratum corneum(SC) inhibits t,ransepidermal water loss and makes a permeability barrier against foreign materials, so strategies to overcome relative impermeability of the SC is very important in transdermal...
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The Effects of Testosterone on Skin Barrier

Bak H, Choi EH, Lee KG, Hong SP, Lee SH, Ahn SK

  • KMID: 1590919
  • Korean J Dermatol.
  • 2005 Feb;43(2):167-176.
BACKGROUND: Although there are no known gender-related differences in permeability barrier function in adults, estrogen accelerates whereas testosterone retards barrier development in fetal skin. However, there have been few studies...
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SDS-PAGE Analysis of Red Cell Membrane Proteins in Hereditary Hemolytic Anemia

Lee YK, Cho HI, Park SS, Ra E, Chang YH, Hur M, Lee YJ, Shin HY, Ahn HS

  • KMID: 1533972
  • Korean J Hematol.
  • 1999 Nov;34(4):559-567.
BACKGROUND: Red cell membrane is a lipid bilayer laminated by the membrane cytoskeleton at the surface of inner monolayer. A class of hemolytic anemia, such as hereditary spherocytosis (HS) or...
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The Changes of Epidermal Lipid and Calcium in the Lesion of Skin Tumor and Non-tumor of Hairless Mice Induced by Vinyl Carbamate Epoxide and TPA

Ahn SK, Bak H, Chun SH, Jeon SY, Choi EH, Lee SJ, Lee SH

  • KMID: 2302794
  • Korean J Dermatol.
  • 2004 Oct;42(10):1304-1312.
BACKGROUND: Chemically induced epidermal carcinogenesis is usually divided into two stages, the initiation and promotion. The initiation involves conversion of some epidermal cells into latent neoplastic cells and the promotion...
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Magnetic Bionanoparticle Enhances Homing of Endothelial Progenitor Cells in Mouse Hindlimb Ischemia

Kang HJ, Kim JY, Lee HJ, Kim KH, Kim TY, Lee CS, Lee HC, Park TH, Kim HS, Park YB

BACKGROUND AND OBJECTIVES: Poor homing efficiency is one of the major limitations of current stem cell therapy. Magnetic bionanoparticles (MPs) obtained from Magnetospirillum sp. AMB-1 have a lipid bilayer membrane...
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The Effect of High Dose Systemic Steroid and its Long-term Usage on the Skin Barrier of Hairless Mice

Lee JW, Bak H, Ahn SK, Choi EH, Lee SH

  • KMID: 2302913
  • Korean J Dermatol.
  • 2004 Mar;42(3):281-289.
BACKGROUND: Prolonged exposure of topical and systemic corticosteroid to skin can result in well-recognized cutaneous abnormalities including cutaneous atrophy, easy bruisibility, increased skin fragility, and increased risk of infection. Skin...
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