Korean J Dermatol.  1998 Oct;36(5):820-826.

Barrier Rcovery after Topically Applied Desoxymethasone Ontment, Vaseline and Hydrobase on Benzalkonium Chloride-irritated Hairless Mice Skin

• Ahn SK
• Choi EH
• Shaojun J
• Hwang SM
• Lee SH

Abstract

BACKGROUND: Topical irritants disrupt the cutaneous permeability barrier through the removal of stratum comeum lipids. This perturbation of barrier integrity stimulates a variety of homeostatic repair responses that ultimately result in the normalization of bamer function. Object To measure the effect of desoxymethasone ointment, vaseline and hydrobase on the barrier recovery of benzalkonium chloride (BKC) imtated skin.
MATERIALS AND METHODS
The left flank skin of 2-3 monthold hairless mice was treated with BKC and then desoxymethasone ointment, vaseline and hydrobase were applied. Transepidermal water loss (TEWL) was checked after 0, 3, 6, 9, 12, 15, 18, 21 and 24 hours. Electron microscopic examination was performed after 3 and 24 hours after desoxymethasone, vaseline and hydrobase had been applied.
RESULTS
The recovery of TEWL was most prominantly observed in the desoxymethasone ointment treated group followed by vaseline and hydrobase. Electron microscopic examination using ruthenium tetroxide fixation revealed that secretion and numbers of lamellar bodies and complete formatice of lipid bilayers were most prominent at desoxymethasone ointment and vaseline treated group.
CONCLUSION
Desoxymethasone ointment, vaseline and hydrobase can be good agents in improving bamer recovery after exposure to irritant material.

MeSH Terms

Animals
Benzalkonium Compounds*
Desoximetasone*
Irritants
Lipid Bilayers
Mice
Mice, Hairless*
Permeability
Petrolatum*
Ruthenium
Skin*
Benzalkonium Compounds
Desoximetasone
Irritants
Lipid Bilayers
Petrolatum
Ruthenium