Korean J Physiol Pharmacol.  2013 Aug;17(4):253-257. 10.4196/kjpp.2013.17.4.253.

Decreasing Effect of Lidocaine.HCl on the Thickness of the Neuronal and Model Membrane

• Park SM
• Park JS
• Kim JH
• Baek JH
• Yoon TG
• Lee DK
• Ryu WH
• Chung IK
• Sohn UD
• Jang HO
• Yun I

• KMID: 2285458
• DOI: http://doi.org/10.4196/kjpp.2013.17.4.253

Abstract

This study examined the mechanism of action of a local anesthetic, lidocaine.HCl. Energy transfer between the surface fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid, and the hydrophobic fluorescent probe, 1,3-di(1-pyrenyl) propane, was used to determine the effect of lidocaine.HCl on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex, and liposomes of the total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. The thickness (D) of the intact SPMV, SPMVTL and SPMVPL were 1.044+/-0.008, 0.914+/-0.005 and 0.890+/-0.003 (arbitrary units, n=5) at 37degrees C (pH 7.4), respectively. Lidocaine.HCl decreased the thickness of the neuronal and model membrane lipid bilayers in a dose-dependent manner with a significant decrease in the thickness, even at 0.1 mM. The decreasing effect of lidocaine.HCl on the membrane thickness might be responsible for some, but not all of its anesthetic action.

Keyword

Fluorescence quenching technique; Lidocaine.HCl; Membrane thickness; Model membranes; Neuronal membranes

MeSH Terms

Anilino Naphthalenesulfonates
Cell Membrane
Cerebral Cortex
Energy Transfer
Lipid Bilayers
Liposomes
Membranes
Neurons
Phospholipids
Propane
Anilino Naphthalenesulfonates
Lipid Bilayers
Liposomes
Phospholipids
Propane

Figure

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  Fig. 1 Effect of lidocaine·HCl on the thickness of the SPMV (A), SPMVTL (B) and SPMVPL (C). The D (a measure of bilayer thickness) values were calculated using eq. 1 and are expressed in arbitrary units (a.u.). Fluorescence measurements were performed at 37℃ (pH 7.4). Each point represents the mean±SEM of 5 determinations. An asterisk and double asterisks denote p<0.05 and p<0.01, respectively, compared to the control using a Student's t-test.

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