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New DNA Extraction Method for Diagnosis of Tuberculosis by Polymerase Chain Reaction

Seo DY, Son HC, Kim SH

  • KMID: 2039325
  • Korean J Clin Pathol.
  • 1997 Feb;17(1):109-119.
BACKGROUND: To assess the clinical utility of new DNA extraction method, the authors attempted PCR using mycobacterial DNA extracted by Chelex 100 ion exchange resin method for 63 clinical samples...
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Purification and Characterization of an Extracellular Alkaline Protease from Aspergillus niger C-15

Kim JD

An alkaline protease produced by Aspergillus niger C-15 was purified and characterized. The enzyme was purified 19.41-fold with a specific activity of 74150 U/mg and a recovery of 34.4% by...
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A Case of Red Blood Cell Exchange Transfusion in a Patient with Hemoglobin S/beta-Thalassemia

Choi R, Song J, Jung HK, Kim S, Jung CW, Park HD, Ki CS, Kang ES, Kim DW

  • KMID: 1424277
  • Korean J Blood Transfus.
  • 2012 Dec;23(3):256-261.
Sickle cell disease and beta-thalassemia are caused by abnormal hemoglobin (Hb) derived from mutation of the HBB gene encoding beta-globin. Compound heterozygous status for both mutations results in Hb S/beta-thalassemia...
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Development of Urine CRM for the quality Control of Heavy Matal Determinations

Park CJ, Cho KH, Suh JK

  • KMID: 1924139
  • Korean J Occup Environ Med.
  • 1995 Oct;7(2):235-248.
Urine certified reference material (CRM) has been developed to help clinical labolatories control analytical accuracy. Two levels of freeze-dried urine were prepared. The low level CRM was made from normal...
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Purification and Characterization of Intracellular Cellulase from Aspergillus oryzae ITCC-4857.01

Begum MF, Absar N

  • KMID: 2408782
  • Mycobiology.
  • 2009 Jun;37(2):121-127.
Purification and characterization of intracellular cellulase produced by A. oryzae ITCC-4857.01 are reported. The enzyme was purified by ion-exchange chromatography using DEAE-cellulose followed by Gel filtration. The purification achieved was...
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Effects of Sample Pretreatment in Amino Acid Analysis

Kim MH, Moon HR

  • KMID: 2300485
  • Korean J Clin Pathol.
  • 2001 Feb;21(1):34-39.
BACKGROUND: Measurements of the concentrations of free amino acids in the blood are used as useful biochemical indicators. The sample pretreatments, including anticoagulant selection and deproteinization, are important steps in...
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Isolation and Identification of the Antigen Recognized by Human Cytomegalovirus Specific Monoclonal Antibody SCMVM 34

Park CG, Kook YH, Cha CY, Hwang ES, Lim DG, Seoh JY, Park JW, Jong HS

  • KMID: 1540528
  • J Korean Soc Microbiol.
  • 1997 Jun;32(3):325-334.
Human cytomegalovirus (HCMV)-specific monoclonal antibody, SCMVM 34, recognizes early antigen confined to the nucleus of HCMV-infected cells. This study was performed to identify the antigen reactive to SCMVM 34 with...
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Cholestyramine Use for Rapid Reversion to Euthyroid States in Patients with Thyrotoxicosis

Ha J, Jo K, Kang B, Kim MH, Lim DJ

Cholestyramine (CS) is an ion exchange resin, which binds to iodothyronines and would lower serum thyroid hormone level. The use of CS added to conventional antithyroid drugs to control thyrotoxicosis...
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An Emerging Diabetes Mellitus Diagnosis Modality: HbA1c

Seo HA, Lee IK

No abstract available.
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Antioxidant Assay of Extracted Fractions Xanthium Strumarium L Using Lens Protein Crosslink Activity

Lee SJ, Lee KW, Chung YS, Hong EK, Lee JH, Wee WR, Joo CK

  • KMID: 2109323
  • J Korean Ophthalmol Soc.
  • 2002 Apr;43(4):751-756.
PURPOSE: To elucidate the anti-oxidant effect of extract fractions from Xanthium strumarium L. on lens protein by crosslinking assay. METHODS: [(1 4)C] N-formyl-lysine was synthesized and purified by ion exchange chromatography....
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Production of Monoclonal Antibody to Polychlorinated Biphenyl Induced Cytochrome P-450 LMII in Rat Liver

Kim JH, Kim JR, Lee KY

Cytochrome P-450 (CP-450) is one of the three components of the liver microsomal enzyme system which hydroxylates fatty acids, hydrocarbons and a variety of drugs and other foreign compounds. Female...
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Rapid Extraction of DNA using Ion Exchange Resin for Early Detection ofMycobacterium tuberculosis by the Polymerase Chain Reaction

Kim CM, Park SK, Shon MH, Kim Y, Song SD, Jun ES, Son HC, Jung BS

  • KMID: 2317970
  • Tuberc Respir Dis.
  • 1996 Feb;43(1):30-37.
BACKGROUND: The extraction methods of DNA from clinical samples are the major obstacle to use the PCR(Polymerase Chain Reaction) in routine labortary for early detection of M. tuberculosis. We tried...
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Binding of B Cell - Derived Autocrine Growth Factor to Hemoglobin

Park JS, Choi IW, Shin YM

  • KMID: 2083011
  • Korean J Immunol.
  • 1998 Mar;20(1):25-30.
Normal human B cells produce autocrine growth factor in response to Staphylococcus aureus Cowan I strain (SAC). However, the functional role and molecular nature of the B cell derived-B cell...
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Molecular pharmacological interaction of phenylbutazone to human neutrophil elastase

Kang K

  • KMID: 1849847
  • Korean J Physiol Pharmacol.
  • 1998 Jun;2(3):385-393.
Human neutrophil elastase (HNElastase, EC 3.4.21.37), a causative factor of inflammatory diseases, was purified by Ultrogel AcA54 gel filtration and CM-Sephadex ion exchange chromatography. HNElastase was inhibited by phenylbutazone in a concentration dependent...
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Purification and Characterization of a Keratinase from a Feather-Degrading Fungus, Aspergillus flavus Strain K-03

Kim JD

  • KMID: 2312534
  • Mycobiology.
  • 2007 Dec;35(4):219-225.
A keratinolytic enzyme secreted by Aspergillus flavus K-03 cultured in feather meal basal medium (FMBM) containing 2% (w/v) chicken feather was purified and characterized. Keratinolytic enzyme secretion was the maximal...
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Analysis of the Chemical Constituents of Agaricus brasiliensis

Cho SM, Jang KY, Park HJ, Park JS

This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty...
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Isolation of a Specific Antigen from the Triton X-100 Soluble Protein (TSP) Of Mycobacterium tuberculosis

Paik TH, Kwon HS, Lee S, Lee JS, Jo EK, Kim HJ, Lee M, Yoo YC, Park JK

  • KMID: 1579120
  • J Bacteriol Virol.
  • 2004 Dec;34(4):273-282.
Tremendous efforts have been made to develop better vaccines and diagnostic markers for the effective control of tuberculosis. Recently, we reported that the Triton X-100 soluble protein (TSP) of Mycobacterium...
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Visualization of the Gastric Calcification due to Cancer on Tc-99m DPD and Abdominal CT Images

Jeong YJ, Kang DY

  • KMID: 1516860
  • Korean J Nucl Med.
  • 2004 Oct;38(5):344-346.
A 69-year-old woman was presented with progressed dysphagia, gastric soreness and weight loss during 2 months. She was performed abdomen x-ray, EGDS and abdomen CT. Abdomen x-ray demonstrated punctuate calcification...
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One Year result of Dermatophagoides farinae antigen - speciffic autologous antibody complex immunotherapy in respiratory allergic patients sensitized to house dust mite

Park JW, Hong CS, Yun YY, Ko SH, Kim CW, Kim KS

  • KMID: 2054843
  • J Asthma Allergy Clin Immunol.
  • 1999 Aug;19(4):566-575.
BACKGROUND: Although good clinical effects have been reported, immunotherapy with house dust mite ( HDM ) antigen - autologous specific antibody complex ( IC - IT ) is not yet...
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Isolation and characterization of brain-specific transglutaminases from rat

Kwak SJ, Kim SY, Kim YS, Song KY, Kim IG, Park SC

  • KMID: 755006
  • Exp Mol Med.
  • 1998 Dec;30(4):177-185.
The relevance of transglutaminases with neural function and several disorders has been emphasized recently. Especially, many polypeptides associated with neurodegenerative diseases are suggested to be putative transglutaminase substrates such as...
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