Yeungnam Univ J Med.  1986 Dec;3(1):103-110. 10.12701/yujm.1986.3.1.103.

Production of Monoclonal Antibody to Polychlorinated Biphenyl Induced Cytochrome P-450 LMII in Rat Liver

• Kim JH
• Kim JR
• Lee KY

• KMID: 2373871
• DOI: http://doi.org/10.12701/yujm.1986.3.1.103

Abstract

Cytochrome P-450 (CP-450) is one of the three components of the liver microsomal enzyme system which hydroxylates fatty acids, hydrocarbons and a variety of drugs and other foreign compounds. Female Balb/c mice were immunized with purified polychlorinated biphenyl (PCB) - induced CP-450 LMII. The spleen cells derived from immunized mice were fused with SP2 myeloma cells using polyethylene glycol (PEG 3500). The hybrid cells were selected by hypoxanthine-aminopterin-thymidine (HAT) medium and the culture fluid were screened by enzyme-linked immunosorbent assay to CP450 LMII. The hybrid cells(×107) were inoculated into intraperitoneal cavity of Balb/c mice for the purpose of production of ascetic fluids. Monoclonal antibody (Mab) was purified from ascitic fluid by DEAE cellulose ion exchange chromatography and I¹²âµ labeled Mab was also confirmed by autoradiography and SDS-polyacrylamide gel electrophoresis (MW:55,000 and 110,000)

MeSH Terms

Animals
Ascitic Fluid
Autoradiography
Chromatography, Ion Exchange
Cytochrome P-450 Enzyme System*
Cytochromes*
DEAE-Cellulose
Electrophoresis
Enzyme-Linked Immunosorbent Assay
Fatty Acids
Female
Humans
Hybrid Cells
Hydrocarbons
Liver*
Mice
Polyethylene Glycols
Rats*
Spleen
Cytochrome P-450 Enzyme System
Cytochromes
DEAE-Cellulose
Fatty Acids
Hydrocarbons
Polyethylene Glycols