Mycobiology.  2008 Mar;36(1):50-54. 10.4489/MYCO.2008.36.1.050.

Analysis of the Chemical Constituents of Agaricus brasiliensis

• Cho SM
• Jang KY
• Park HJ
• Park JS

• KMID: 2052888
• DOI: http://doi.org/10.4489/MYCO.2008.36.1.050

Abstract

This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by 13C-NMR. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By 13C-NMR analysis, the immuno-stimulating substance was identified as beta-(1-->3) (1-->6)-glucan, composed of a backbone with (1-->3)-linked D-glucopyranosyl residues branching a (1-->6)-linked D-glucopyranosyl residue. The beta-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

Keyword

Agaricus blasiliensis; Chemical composition; Immuno-stimulating substance

MeSH Terms

Agaricus
Amino Acids
Arginine
B-Lymphocytes
Carbohydrates
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
DEAE-Cellulose
Ethanol
Fatty Acids
Fumarates
Glutamic Acid
Glycine
Linoleic Acid
Mannitol
Nutritive Value
Water
Amino Acids
Arginine
Carbohydrates
DEAE-Cellulose
Ethanol
Fatty Acids
Fumarates
Glutamic Acid
Glycine
Linoleic Acid
Mannitol
Water