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Cerebellar encephalopathy from diminazene aceturate (beneril) toxicity in a dog

Han D, Yoon WK, Hyun C

  • KMID: 1726752
  • Korean J Vet Res.
  • 2014 Sep;54(3):193-196.
A 2-year old castrated male Alaskan malamute was referred with primary complaints of marked anemia, hemeglobinuria and depression. Laboratory tests revealed canine babesiois with severe anemia. The dog was treated...
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Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma

Staroverov SA, Sidorkin V, Fomin AS, Shchyogolev SY, Dykman LA

In this work, we used a preparation of diminazene, which belongs to the group of aromatic diamidines. This compound acts on the causative agents of blood protozoan diseases produced by...
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Stimulatory Effects of Ginsan on the Proliferation and Viability of Mouse Spleen Cells

Ko EJ, Joo HG

Ginsan is an acidic polysaccharide purified from Panax ginseng, a famous oriental herb. Although a variety of biological activities of ginsan have been studied, the effects of ginsan on spleen...
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Inadvertent Staining of the Vitreous with Trypan Blue During Cataract Surgery

Lee MY, Lee HS, Joo CK

PURPOSE: We report a case of inadvertent staining of the vitreous with Trypan Blue during cataract surgery. CASE SUMMARY: We performed cataract surgery in a 44-year-old male patient on an eye...
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Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae

Jo WS, Bae SH, Choi SY, Park SD, Yoo YB, Park SC

  • KMID: 2312569
  • Mycobiology.
  • 2010 Mar;38(1):74-77.
To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol...
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Trypan Blue-Assisted Measurements of Anatomical Positions of the Superior Rectus Muscle and Superior Oblique Muscle in Enucleated Eyes

Shin YT, Chun BY

  • KMID: 2338274
  • J Korean Ophthalmol Soc.
  • 2012 Oct;53(10):1488-1492.
PURPOSE: To recognize the anatomical positions of the superior oblique muscle in enucleated eyes using trypan blue. METHODS: Twenty-two surgically-enucleated eyes of 11 bodies were studied. The shortest distance from the...
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Immunostimulatory Effects of beta-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes

Kim JM, Joo HG

We investigated the effects of beta-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan...
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Optimal Media Conditions for the Detection of Extracellular Cellulase Activity in Ganoderma neo-japonicum

Jo WS, Park HN, Cho DH, Yoo YB, Park SC

  • KMID: 2312629
  • Mycobiology.
  • 2011 Jun;39(2):129-132.
To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the...
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Clindamycin-doxycycline-metronidazole combination therapy in a refractory canine babesiosis case

An HM, Song JH, An SJ, Yu DH, Han D, Kim YJ, Jung DI

An 8-year-old castrated male Maltese dog (patient) was referred to our institute with refractory canine babesiosis. The patient had previously responded to conventional treatment with atovaquone and azithromycin; however, anemia...
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Role of Clusterin and Tumor Necrosis Factor Receptors on the Apoptosis of Prostate Cancer Cells

Joo KJ

PURPOSE: In prostate cancer, the anti-apoptotic mechanism of sulfated glycoprotein-2 (clusterin) against tumor necrosis factor-alpha (TNF-alpha) receptors and the action of type 2 TNF-alpha receptor (TNFR2) were investigated. MATERIALS AND METHODS:...
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Assessment of Cell Viability in Umbilical Cord Blood before Cryopreservation

Yi DY, Huh JY, Kang MS

  • KMID: 2090012
  • Korean J Blood Transfus.
  • 2010 Aug;21(2):140-147.
BACKGROUND: The viability of cord blood is an important measure of product quality. Trypan blue (TB) stain is the most commonly and conveniently used method to measure the viability of...
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Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum

Jo WS, Bae SH, Cho DH, Park SD, Yoo YB, Park SC

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the...
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Immunostimulatory effects of BCG-CWS on the proliferation and viability of mouse spleen cells

Lee CW, Ko EJ, Joo HG

  • KMID: 1435819
  • Korean J Vet Res.
  • 2012 Jun;52(2):89-97.
Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been tried to use for immunotherapy. The CWS of Mycobacterium bovis bacillus Calmette-Guerin (BCG-CWS) was studied as...
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Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells

Kim HS, Kim KH, Kim SH, Kim YS, Koo KT, Kim TI, Seol YJ, Ku Y, Rhyu IC, Chung CP, Lee YM

PURPOSE: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro. METHODS: Mixed cell cultures consisting...
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Effects of Knockout Serum Replacement in the Culture Medium on the Proliferation of Porcine Fetal Fibroblasts In Vitro

Kim EJ, Park JJ, Choi YJ, Park SK, Roh S

  • KMID: 1719926
  • Int J Oral Biol.
  • 2010 Mar;35(1):1-5.
Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum. However, there are potential disadvantages of the use...
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Both R(-)- and S(+)-Citalopram Attenuate Neuronal Apoptosis in Murine Cortical Cell Culture

Eu MY, Chung YK, Noh JS

  • KMID: 2287390
  • Korean J Psychopharmacol.
  • 2009 Nov;20(6):301-306.
OBJECTIVE: Several lines of evidence have shown that neuronal injury might represent a possible pathway to mood disorders. The present study was performed to examine the possibility that the neuroprotective...
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Difference in Viability of CD34+Cells in Cryopreserved Cord Blood According to Evaluation Methods

Ahn MS, Eom YW, Park JS, Choi JH, Kang SY, Lee HW, Yang MS, Kim HE, Jang IK, Lee JE, Kim YJ, Kim HC, Jeong SH

BACKGROUND: On performing umbilical cord blood (UCB) transplantation, faster engraftment may lead better clinical outcome. Because transplanted viable cell count in UCB is related to the engraftment, accurate evaluation of...
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The effects of propofol on neurotoxicity induced by alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid in rat mixed cortical cultures

Seo MS, Park SY, Kim KS, Moon BK, Kim JS, Lee SY

BACKGROUND: The pattern of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated neurotoxicity (necrosis vs apoptosis) and the neuroprotective effect of propofol on AMPA-mediated neurotoxicity are still unclear. METHODS: Thirteen-day-old primary rat mixed cortical cultures were...
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Cryopreservation of Collected Peripheral Blood Hematopoietic Stem Cell Product with 5% DMSO by Adding Nontoxic Natural Cryoprotectants

Hamm JY, Shon YH, Suh JS

  • KMID: 1442287
  • Korean J Blood Transfus.
  • 2011 Aug;22(2):89-98.
BACKGROUND: Cryopreservation of hematopoietic stem cells has become an important process due to the therapeutic protocol, which includes stem cell transplantation after chemotherapy, for many hematological malignancies. The conventional medium...
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Capsaicin induced apoptosis and the enhanced anticancer effect of anticancer drugs in cancer cells

Kim SY, Lee YJ, Park EH, Yi HK, Jo DS, Kim JS, Hwang PH

PURPOSE: Capsaicin, the major pungent ingredient in red pepper, has long been used in spices and food additives. It has been recently shown to induce apoptosis in several cell lines...
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