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Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle

Selahi F, Namavari M, Hosseini M, Mansourian M, Tahamtan Y

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of...
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Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Garcia A, Martinez R, Benitez-Medina JM, Risco D, Garcia WL, Rey J, Alonso JM, de Mendoza JH

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green...
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An atypical case of respiratory actinobacillosis in a cow

Angelo P, Alessandro S, Noemi R, Giuliano B, Filippo S, Marco P

A not pregnant 4-year-old Jersey cow was presented with the sudden appearance of respiratory noise, nasal discharge and moderate respiratory difficulty. Upon physical examination a snoring-like noise, extended head and...
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Diagnosis of bovine cryptosporidiosis by indirect immunofluorescence assay using monoclonal antibody

Wee SH, Lee CG, Joo HD

Two hybridoma cell lines against Cryptosporidium parvum oocysts (VRI- CN91) were produced. The isotype of these 2 monoclonal antibodies (mAbs) was IgG2b (1E7.2) and and IgM (C6). Enzyme immuno-transfer blotting...
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Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

Reyes AW, Simborio H, Hop HT, Arayan LT, Kim S

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but...
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A serological study of severe fever with thrombocytopenia syndrome using a virus neutralization test and competitive enzyme-linked immunosorbent assay

Lee H, Kim EJ, Cho IS, Song JY, Choi JS, Lee JY, Shin YK

Severe fever with thrombocytopenia syndrome (SFTS) is caused by the SFTS virus (SFTSV). The SFTSV appears to have a wide host range, as SFTSV-positive ticks have been isolated from both...
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Performance of the SD Bioline TB Ag MPT64 Rapid test for quick confirmation of Mycobacterium bovis isolates from animals

Byeon HS, Ji MJ, Kang SS, Kim SW, Kim SC, Park SY, Kim G, Kim J, Cho JE, Ku BK, Kim JM, Jeon BY

Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing...
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