J Vet Sci.  2015 Mar;16(1):31-35. 10.4142/jvs.2015.16.1.31.

Performance of the SD Bioline TB Ag MPT64 Rapid test for quick confirmation of Mycobacterium bovis isolates from animals

• Byeon HS
• Ji MJ
• Kang SS
• Kim SW
• Kim SC
• Park SY
• Kim G
• Kim J
• Cho JE
• Ku BK
• Kim JM
• Jeon BY

• KMID: 2160818
• DOI: http://doi.org/10.4142/jvs.2015.16.1.31

Abstract

Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals.

Keyword

animals; Mycobacterium bovis; SD MPT64 Rapid test

MeSH Terms

Animals
Cattle
Cattle Diseases/*diagnosis/microbiology
*Deer
Immunochromatography/methods/*veterinary
Mycobacterium bovis/classification/*isolation & purification
Sensitivity and Specificity
Tuberculosis/diagnosis/microbiology/*veterinary

Figure

• 

  Fig. 1 Detection limit of the SD MPT64 Rapid test for Mycobacterium (M.) bovis. The test was used to analyze a series of diluted suspensions of M. bovis AN5 cultured in Middlebrook 7H9 broth with enrichment. A representative image of three tests is shown. The results were interpreted as positive (+), negative (-), or weak (±). NC, negative control; 1, 2.1 × 103 CFU/mL of M. bovis; 2, 4.2 × 103 CFU/mL of M. bovis; 3, 8.5 × 103 CFU/mL of M. bovis; 4, 1.7 × 104 CFU/mL of M. bovis; 5, 3.4 × 104 CFU/mL of M. bovis; 6, 6.8 × 104 CFU/mL of M. bovis; 7, 1.3 × 105 CFU/mL of M. bovis; 8, 2.7 × 105 CFU/mL of M. bovis; 9, 5.4 × 105 CFU/mL of M. bovis; 10, 1.0 × 106 CFU/mL of M. bovis; 11, 2.0 × 106 CFU/mL of M. bovis.

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