Korean J Clin Microbiol.  2011 Mar;14(1):36-38. 10.5145/KJCM.2011.14.1.36.

A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay

• Yoon CE
• Hong YJ
• Lee JK
• Chang YH
• Hong SI

• KMID: 1449497
• DOI: http://doi.org/10.5145/KJCM.2011.14.1.36

Abstract

Mycobacterium tuberculosis complex (MTBC) is discriminated from non-tuberculous mycobacteria (NTM) via an immunochromatographic assay (ICA) which is based on the reactions of monoclonal antibodies against MPT64, one of the predominant proteins excreted by MTBC. Recently, the authors of the present study discovered SD TB-negative Mycobacterium tuberculosis strains. In addition, sequence analysis of the mpt64 genes in these strains was performed and showed a deletion of 63 bp from nucleotides 196 to 258. In cases of MPT64-negative mycobacterium, the authors recommend performing TB PCR for correct diagnosis.

Keyword

Mycobacterium tuberculosis; mpt64 gene; Mutation

MeSH Terms

Antibodies, Monoclonal
Immunochromatography
Mycobacterium
Mycobacterium tuberculosis
Nucleotides
Polymerase Chain Reaction
Proteins
Sequence Analysis
Antibodies, Monoclonal
Nucleotides
Proteins

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