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Correlation between GenoType MTBDRplus Assay and Phenotypic Susceptibility Test for Prothionamide in Patients with Genotypic Isoniazid Resistance

Lee JH, Jo KW, Shim TS

BACKGROUND: The purpose of this study was to analyze the relationship between the gene mutation patterns by the GenoType MTBDRplus (MTBDRplus) assay and the phenotypic drug susceptibility test (pDST) results...
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A Rapid Radicle Assay for Prescreening Antagonistic Bacteria Against Phytophthora capsici on Pepper

Chang SH, Kwack MS, Kim YS, Lee JY, Kim KD

  • KMID: 2052970
  • Mycobiology.
  • 2001 Dec;29(4):218-223.
A rapid radicle assay for prescreening antagonistic bacteria to Phytophthora capsici, causal agent of Phytophthora blight of pepper was developed. Sixty-four bacterial strains with in vitro antifungal activity selected out...
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MTT and Neutral red Biologic Assay for Human Bladder Cancer Cell Line(TCC-SuP) and Androgen Stimulated Cell Line of Mouse (SC-115): In Vitro Chemosensitivity Test

Kim BW, Kim DY, Chung SK, Chang SK

  • KMID: 2289661
  • Korean J Urol.
  • 1990 Oct;31(5):635-640.
To overcome the limitations of clonogenic assay (low plating efficiencies, clumping artifacts, long assay duration), we performed chemosensitivity test using MTT and neutral red biologic assay on SC-115 (androgen stimulated...
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Mycoherbicidal Potential of Phaeoacremonium italicum, A New Pathogen of Eichhornia crassipes Infesting Harike Wetland, India

Singh B, Saxena S, Meshram V, Kumar M

Mycoherbicides are exclusive biotechnology products which offer a non-chemical solution to control noxious weeds on the land as well as aquatic in systems, viz a viz saving environment from hazardous...
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Frequency and Type of Disputed rpoB Mutations in Mycobacterium tuberculosis Isolates from South Korea

Jo KW, Lee S, Kang MR, Sung H, Kim MN, Shim TS

BACKGROUND: A disputed rpoB mutation is a specific type of rpoB mutation that can cause low-level resistances to rifampin (RIF). Here, we aimed to assess the frequency and types of...
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Serum Soluble Epidermal Growth Factor Receptor Level Increase in Patients Newly Diagnosed with Type 2 Diabetes Mellitus

Kim JM, Choung S, Joung KH, Lee JH, Kim HJ, Ku BJ

We analyzed circulating soluble epidermal growth factor receptor (sEGFR) levels in humans. Serum sEGFR levels were higher in subjects with newly diagnosed type 2 diabetes mellitus compared with controls. Serum...
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Mycoflora and Mycotoxins of Cereal Grains in Delta, Egypt

Soliman HM

  • KMID: 2312410
  • Mycobiology.
  • 2003 Dec;31(4):183-190.
Five cereal grains (wheat, barley, rice, maize and sorghum) were collected from three Egyptian provinces known to be grain producers (Daqahlia, Gharbia and Kafer el-Sheikh). Two species of Alternaria (A....
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Cotinine-conjugated aptamer/anti-cotinine antibody complexes as a novel affinity unit for use in biological assays

Park S, Hwang D, Chung J

  • KMID: 1389735
  • Exp Mol Med.
  • 2012 Sep;44(9):554-561.
Aptamers are synthetic, relatively short (e.g., 20-80 bases) RNA or ssDNA oligonucleotides that can bind targets with high affinity and specificity, similar to antibodies, because they can fold into unique,...
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Clinical Implication of TSH Receptor Antibody Measurement

Shin DY

Autoantibodies directed against the thyrotropin receptor have been well known to be an important pathogenesis of Graves' disease. However, the diagnosis and management of Graves' disease are still mainly dependent...
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Establishment of Rapid Detection Methods for Bovine Viral Diarrhea Virus(Pestivirus) in Attenuated Animal Viral Vaccines by RT-PCR and Nested PCR

An DJ, Kim BH, Jeon WJ, Jung BY, Yi CH, Park BK, Chung GS

  • KMID: 1574173
  • J Bacteriol Virol.
  • 2005 Sep;35(3):273-281.
Bovine viral diarrhea virus (BVDV) of the genus Pestivirus is known as a common contaminant of cell culture-derived vaccines. Hog cholera virus (HCV), which is also of the genus Pestivirus...
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Mushrooms Collected from Deogyu Mountain, Muju, Korea and Their Antioxidant Activity

Kim SE, Lee IK, Jung YA, Yeom JH, Ki DW, Lee MS, Song JG, Jin YJ, Seok SJ, Yun BS

Mushrooms collected from Deogyu mountain, Korea, in 2011, were identified as four classes, four orders, 13 families, 22 genera, and 33 species. In particular, agaricales was most abundant and comprised...
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Detection of Botulinum Neurotoxin Type A by In Vitro Bioassay Based on Endopeptidase Activity

Kim YJ, Baek JH, Kim JH, Kim BS, Rhie GE, Yoo CK, Shin NR

Botulinum neurotoxin type A (BoNT/A) is a metalloprotease that cleaves SNAP-25 (synaptosome-associated protein of 25 kDa), a specific cellular protein essential for neurotransmitter release. As well as mouse bioassay to...
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Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR

Wang Y, Wang G, Zhang D, Yin H, Wang M

  • KMID: 1792151
  • Korean J Parasitol.
  • 2013 Oct;51(5):573-577.
A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected...
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Validation of a Multiplexed Opsonophagocytic Assay for 11 Additional Pneumococcal Serotypes and Its Application to Functional Antibody Evaluation Induced by Pneumococcal Polysaccharide Vaccine

Cha J, Kim HW, Lee JH, Lee S, Kim KH

BACKGROUND: Various pneumococcal vaccines have been evaluated for immunogenicity by opsonophagocytic assay (OPA). A multiplexed OPA (MOPA) for 13 pneumococcal serotypes was developed by Nahm and Burton, and expanded to...
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Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease

Lee JI, Jang HW, Kim SK, Choi JY, Kim JY, Hur KY, Kim JH, Min YK, Chung JH, Kim SW

BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and...
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Screening for Antifungal Endophytic Fungi Against Six Plant Pathogenic Fungi

Park JH, Park JH, Choi GJ, Lee SW, Jang KS, Choi YH, Cho KY, Kim JC

A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 11 locations in Korea. Their antifungal activities were screened in vivo by antifungal bioassays...
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Usefulness of expression of protease as biologic marker in 5-FU resistant human colon cancer cell line HT29

Cho M, Choi SR, Lee JI, Lee CJ, Kim YS

  • KMID: 2251351
  • Korean J Med.
  • 1999 Sep;57(3):323-332.
There have been many reports that colon cancer responds poorly to chemotherapy. Several classes of matrix metalloproteinases(MMPs) have been implicated in the process of invasion of epithelial and endothelial basement membranes in...
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Thyroid Stimulating Antibody Assay with Chinese Hamster Ovary Cells Expressing Human Thyroid Stimulating Hormone (TSH) Receptor; Optimization of Assay Condition

Cho BY, Lee HK, Shong YK, Koh CS, Yi KH, Oh YS, Kim WB

  • KMID: 2462168
  • J Korean Soc Endocrinol.
  • 1995 Dec;10(4):333-346.
We investigated the optimal condition of thyroid stimulating antibody(TSAb) assay using Chinese hamster ovary cells transfected with cDNA of human TSH receptor(TSHr-CHO) stably expressing functional TSH receptors. The extracellular cAMP...
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Scientific rationale and applicability of dose-reponse models for environmental carcinogens

Shin D, Chung Y, Kim J, Lee SG, Hwang M

  • KMID: 2282787
  • Korean J Prev Med.
  • 1996 Mar;29(1):27-42.
This study described methods to predict human health risk associated with exposure to environmental carciongens using animal bioassay data. Also, biological assumption for various dose-response models were reviewed. To illustrate...
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Comparison of Molecular Biologic Methods for Detecting HBV-DNA in the Sera which Showed Both Hepatitis B Surface Antigen and Antibody Positivity

Kim MJ, Kim HS, Kwon OH, Hahn KH

  • KMID: 2146213
  • Korean J Clin Pathol.
  • 1997 Dec;17(6):1124-1136.
BACKGROUND: Serologic markers are used to screen and diagnose the hepatitis B virus infection. In endemic area of hepatitis B, the coexistence of HBsAg and anti-HBs was frequently observed. This...
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