Korean J Urol.  1990 Oct;31(5):635-640.

MTT and Neutral red Biologic Assay for Human Bladder Cancer Cell Line(TCC-SuP) and Androgen Stimulated Cell Line of Mouse (SC-115): In Vitro Chemosensitivity Test

• Kim BW
• Kim DY
• Chung SK
• Chang SK

Abstract

To overcome the limitations of clonogenic assay (low plating efficiencies, clumping artifacts, long assay duration), we performed chemosensitivity test using MTT and neutral red biologic assay on SC-115 (androgen stimulated mouse tumor) and TCC-SuP (human bladder tumor) cell lines. The anticancer agents tested were adriamycin, cisplatin, methotrexate and combination of these drugs. And the following results were obtained ; 1) The growth of two cell lines were inhibited dose-dependently by application of adriamycin, cisplatin and combination of drugs. But methotrexate was not effective on TCC-SuP cell line. 2) The neutral red biologic assay is more convenient and rapid method than MTT assay but staining ability is superior in MTT assay. 3) The MTT and neutral red biologic assay offer advantages in speed, simplicity, cost & safety for the chemosensitivity test and for determining the new regimen.

Keyword

MTT; Neutral red; TCC-SuP; SC-115

MeSH Terms

Animals
Antineoplastic Agents
Artifacts
Biological Assay*
Cell Line*
Cisplatin
Doxorubicin
Humans*
Methotrexate
Mice*
Neutral Red*
Staining and Labeling
Urinary Bladder Neoplasms*
Urinary Bladder*
Antineoplastic Agents
Cisplatin
Doxorubicin
Methotrexate
Neutral Red