CHEMOSENSITIVITY OF CISPLATIN AND 5-FLUOROURACIL ON ORAL SQUAMOUS CELL CARCINOMA CELL LINES

Lee, Jong Hwan; Kim, Myung Jin

Abstract

The oral squamous cell carcinoma is majority of oral cacer and the treated of oral cancer strategies of clinical oncologist. The remission induced by anti-cancer agents is attributable to the action of the anticancer agents on drug sensitive cell populations in the tumor. However is spite of vatiable combination therapy, the majority of aptients with advanced disease relapse, and some of patients reveals clinical problems of resistence to cancer chemotherapeutic agents. The in vitro chemosensitivity test of anticancer agent gives clinical basis in chemotherapy. The semiautomated tetrazolium dye assay(MTT) method is used to measure cytotoxicity, proliferation or activation of mammalian cells with high degree of precision and rapidity. The present study was performed to assess the growth inhibitory effect of 5-fluorouracil and cisplatin in oral squamous cell carcinoma cell line KB and primarily cultured cell oral squamous cell carcinoma patient using tetrazolium dye assay method(MTT). The results are as follows. 1. Cisplatin had dose dependent cytotoxic effect in 24h incubation while 5-fluorouracil had time dependent effect on both cell lines. 2. On KB cell line, IC50 of cisplatin was 0.74 microgram/ml and IC50 of 5-fluorouracil was 1.90 microgram/ml while IC50 of cisplatin and IC50 of 5-fluorouracil on primary culture cell were 0.57 microgram/ml and 2.83 microgram/ml respectively. 3. On KB and primary cultured cell, combined treated group did not show more cytotoxic effect with statistical significancy(p-value=0.067, 0.176 respectively) than cisplatin single treated group but had dilution benefit. 4. Primarily cultured oral squamous cell carcinoma cell carcinoma tumor cells from patient specimen responds to chemotherapeutic agent as response profiles of standard cell line, MTT assay is considered as clinical tool in predicting chemosensitivity.