Mycobiology.  2003 Sep;31(3):179-182. 10.4489/MYCO.2003.31.3.179.

Screening for Antifungal Endophytic Fungi Against Six Plant Pathogenic Fungi

• Park JH
• Park JH
• Choi GJ
• Lee SW
• Jang KS
• Choi YH
• Cho KY
• Kim JC

• KMID: 2052992
• DOI: http://doi.org/10.4489/MYCO.2003.31.3.179

Abstract

A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 11 locations in Korea. Their antifungal activities were screened in vivo by antifungal bioassays after they were cultured in potato dextrose broth and rice solid media. Antifungal activity against plant pathogenic fungi such as Magnaporthe grisea (rice blast), Corticium sasaki (rice sheath blight), Botrytis cinerea (tomato gray mold), Phytophthora infestans (tomato late blight), Puccinia recondita (wheat leaf rust), and Blumeria graminis f. sp. hordei (barley powdery mildew) was determined in vivo by observing the inhibition of plant disease development. Twenty (11.7%) endophytic fungi fermentation broths were able to control, by more than 90%, at least one of the six plant diseases tested. Among 187 liquid broths, the F0010 strain isolated from Abies holophylla had the most potent disease control activity; it showed control values of more than 90% against five plant diseases, except for tomato late blight. On the other hand, fourteen (7.5%) solid culture extracts exhibited potent disease control values of more than 90% against one of six plant diseases. The screening results of this study strongly suggested that metabolites of plant endophytic fungi could be good potential sources for screening programs of bioactive natural products.

Keyword

Antifungal metabolite; Endophytic fungi; In vivo antifungal bioassays; Plant disease; Plant pathogen

MeSH Terms

Abies
Biological Assay
Biological Products
Botrytis
Fermentation
Fungi*
Glucose
Hand
Korea
Lycopersicon esculentum
Magnaporthe
Mass Screening*
Phytophthora infestans
Plant Diseases
Plants*
Solanum tuberosum
Biological Products
Glucose