Mycobiology.  2000 Dec;28(4):190-192.

Screening for In Vitro Antifungal Activity of Soil Bacteria Against Plant Pathogens

• Chang SH
• Lee JY
• Kim KD
• Hwang BK

Abstract

Antifungal bacteria for biological control of plant diseases or production of novel antibiotics to plant pathogens were isolated in 1997 from various soils of Ansung, Chunan, Koyang, and Paju in Korea. Sixty-four bacterial strains pre-screened from approximately 1,400 strains were tested on V-8 juice agar against eight plant pathogenic fungi using in vitro bioassay technique for inhibition of mycelial growth. Test pathogens were Alternaria mali, Colletotrichum gloeosporioides, C. orbiculare, Fusarium oxysporum f. sp. cucumerinum, F. oxysporum f. sp. lycopersici, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. A wide range of antifungal activity of bacterial strains was found against the pathogenic fungi, and strain RC-B77 showed the best antifungal activity. Correlation analysis between inhibition of each fungus and mean inhibition of all eight fungi by 64 bacterial strains revealed that C. gloeosporioides would be best appropriate for detecting bacterial strains producing antibiotics with potential as biocontrol agents for plant pathogens.

Keyword

Antifungal activity; Colletotrichum gloeosporioides; Fungi; Plant pathogen; Screening; Soil bacteria

MeSH Terms

Agar
Alternaria
Anti-Bacterial Agents
Bacteria*
Biological Assay
Chungcheongnam-do
Colletotrichum
Fungi
Fusarium
Gyeonggi-do
Korea
Magnaporthe
Mali
Mass Screening*
Phytophthora
Plant Diseases
Plants*
Rhizoctonia
Soil*
Agar
Anti-Bacterial Agents
Soil