Mycobiology.  2017 Dec;45(4):370-378. 10.5941/MYCO.2017.45.4.370.

Antagonistic Evaluation of Chromobacterium sp. JH7 for Biological Control of Ginseng Root Rot Caused by Cylindrocarpon destructans

• Han JH
• Park GC
• Kim KS

• KMID: 2401067
• DOI: http://doi.org/10.5941/MYCO.2017.45.4.370

Abstract

Cylindrocarpon destructans is an ascomycete soil-borne pathogen that causes ginseng root rot. To identify effective biocontrol agents, we isolated several bacteria from ginseng cultivation soil and evaluated their antifungal activity. Among the isolated bacteria, one isolate (named JH7) was selected for its high antibiotic activity and was further examined for antagonism against fungal pathogens. Strain JH7 was identified as a Chromobacterium sp. using phylogenetic analysis based on 16S rRNA gene sequences. This strain was shown to produce antimicrobial molecules, including chitinases and proteases, but not cellulases. Additionally, the ability of JH7 to produce siderophore and solubilize insoluble phosphate supports its antagonistic and beneficial traits for plant growth. The JH7 strain suppressed the conidiation, conidial germination, and chlamydospore formation of C. destructans. Furthermore, the JH7 strain inhibited other plant pathogenic fungi. Thus, it provides a basis for developing a biocontrol agent for ginseng cultivation.

Keyword

Chromobacterium; Cylindrocarpon destructans; Ginseng root rot

MeSH Terms

Ascomycota
Bacteria
Cellulases
Chromobacterium*
Fungi
Genes, rRNA
Germination
Panax*
Peptide Hydrolases
Plants
Soil
Cellulases
Peptide Hydrolases
Soil