Mycobiology.  2019 Sep;47(3):329-334. 10.1080/12298093.2019.1635425.

Antifungal Activities of Streptomyces blastmyceticus Strain 12-6 Against Plant Pathogenic Fungi

• Kim YJ
• Kim JH
• Rho JY

• KMID: 2470144
• DOI: http://doi.org/10.1080/12298093.2019.1635425

Abstract

Streptomyces blastmyceticus strain 12-6 was isolated from a forest soil sample of Cheonan area on the basis of strong antifungal activities against plant pathogenic fungi. Butanol extracts of the cultural filtrates were active against C. acutatum, C. coccodes, C. gloeosporioides, F. oxysporum, and T. roseum. Active fractions were prepared by thin layer chromatography using silica gel plate; 12-6-2 (R(f) 0.36), 12-6-3 (R(f) 0.44). Scanning electron microscopy showed that the active fractions caused a change in surface texture of fungal spores from smooth surface to wrinkled surface. The lethal effect on the spores of the active fractions varied from 56% to 100%. It was shown that the spores of C. acutatum were more sensitive to the antifungal fractions than the spores of F. oxysporum. Fluorescence staining using TOTO-1 indicated that the antifungal fractions could make the spores more sensitive to the fluorescence dye. Thus, it was suggested that antifungal agents prepared in this study exhibited the antifungal activity by damaging the plasma membrane of both fungal spores and hyphae. Identification of antifungal agents in the active fraction using GC-MS analysis revealed the presence of cyclo-(Leu-Pro) and 9-octadecenamide as major components that have already been known as antifungal substances.

Keyword

Antifungal activity; Streptomyces; plant pathogenic; spore; hyphae

MeSH Terms

Antifungal Agents
Cell Membrane
Chromatography, Thin Layer
Chungcheongnam-do
Fluorescence
Forests
Fungi*
Hyphae
Microscopy, Electron, Scanning
Plants*
Silica Gel
Soil
Spores
Spores, Fungal
Streptomyces*
Antifungal Agents
Silica Gel
Soil