- Serotypes of Streptococcus pyogenes isolated from healthy school children in Kangwon-do
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Kim SJ, Cha SH, Kim EC, Kaplan EL
- KMID: 1422187
- J Korean Med Sci.
- 1994 Feb;9(1):52-56.
- doi: 10.3346/jkms.1994.9.1.52
Seventy-seven Streptococcus pyogenes strains isolated of children of three elementary schools located in Kangwon-do in spring, 1992 were serotyped with M, opacity factor (OF) and T typing antisera. In the... -
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- Molecular Typing in Public Health Laboratories: From an Academic Indulgence to an Infection Control Imperative
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Allerberger F
- KMID: 1120117
- J Prev Med Public Health.
- 2012 Jan;45(1):1-7.
- doi: 10.3961/jpmph.2012.45.1.1
Using three Austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on DNA subtyping. DNA macrorestriction... -
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- Expression of Sme Efflux Pumps and Multilocus Sequence Typing in Clinical Isolates of Stenotrophomonas maltophilia
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Cho HH, Sung JY, Kwon KC, Koo SH
- KMID: 1781436
- Ann Lab Med.
- 2012 Jan;32(1):38-43.
- doi: 10.3343/alm.2012.32.1.38
BACKGROUND: Stenotrophomonas maltophilia has emerged as an important opportunistic pathogen, which causes infections that are often difficult to manage because of the inherent resistance of the pathogen to a variety... -
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- Molecular and Epidemiological Characterization of Carbapenem-Resistant Acinetobacter baumannii in Non-Tertiary Korean Hospitals
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Park S, Kim HS, Lee KM, Yoo JS, Yoo JI, Lee YS, Chung GT
- KMID: 1776935
- Yonsei Med J.
- 2013 Jan;54(1):177-182.
- doi: 10.3349/ymj.2013.54.1.177
PURPOSE: The increasing prevalence and global spread of carbapenem-resistant Acinetobacter baumannii (A. baumannii) has become a serious problem. The aim of this study was to investigate molecular and epidemiological characteristics... -
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- Molecular Evolution and Identification of Yersinia Species by 16S rDNA Analysis
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Chung SI, Choi CS, Kim WY, Song MO, Park CM, Kim KJ, Yu IH
- KMID: 2146895
- J Korean Soc Microbiol.
- 1999 Aug;34(4):337-345.
Sequence information from the ribosomal RNA molecule can be used as a molecular clock. Therefore, 16S rRNA sequences have become a widely used tool in bacterial systematics, bacterial typing and... -
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- Characterization of a Vancomycin-resistant Enterococcus faecium Outbreak Caused by 2 Genetically Different Clones at a Neonatal Intensive Care Unit
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Lee WG, Ahn SH, Jung MK, Jin HY, Park IJ
- KMID: 1781443
- Ann Lab Med.
- 2012 Jan;32(1):82-86.
- doi: 10.3343/alm.2012.32.1.82
In July 2010, we identified an outbreak of vancomycin-resistant enterococci (VRE) in our 26-bed neonatal intensive care unit. We performed an epidemiological investigation after clinical cultures of 2 neonates were... -
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- Dissimilarity of ccrAB gene sequences between methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus among bovine isolates in Korea
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Park YK, Paik YH, Yoon JW, Fox LK, Hwang SY, Park YH
- KMID: 1705560
- J Vet Sci.
- 2013 Sep;14(3):299-305.
- doi: 10.4142/jvs.2013.14.3.299
The sequences of the ccrAB genes from bovine-, canine- and chicken-originating methicillin-resistant Staphylococcus (S.) epidermidis (MRSE) and bovine methicillin-resistant Staphylococcus (S.) aureus (MRSA) were compared to investigate the frequency of... -
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- Three Cases of Moraxella osloensis Meningitis: A Difficult Experience in Species Identification and Determination of Clinical Significance
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Roh KH, Kim CK, Koh E, Kim MS, Yong D, Park SC, Lee K, Chong Y
- KMID: 1778053
- J Korean Med Sci.
- 2010 Mar;25(3):501-504.
- doi: 10.3346/jkms.2010.25.3.501
We had three cases of Moraxella osloensis meningitis. The species identification was impossible by conventional and commercial phenotypic tests. However, we could identify the species using the 16S rRNA gene... -
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- Epidemiological Study of Erythromycin-Resistant Streptococcus pyogenes From Korea and Japan by emm Genotyping and Multilocus Sequence Typing
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Takahashi , Arai K, Lee DH, Koh EH, Yoshida H, Yano H, Kaku M, Kim S
- KMID: 2373491
- Ann Lab Med.
- 2016 Jan;36(1):9-14.
- doi: 10.3343/alm.2016.36.1.9
BACKGROUND: We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST). METHODS: Clinical... -
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- Strain Typing of Mycobacterium tuberculosis Isolates from Korea by Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats
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Yun KW, Song EJ, Choi GE, Hwang IK, Lee EY, Chang CL
- KMID: 1096924
- Korean J Lab Med.
- 2009 Aug;29(4):314-319.
- doi: 10.3343/kjlm.2009.29.4.314
BACKGROUND: Mycobacterium tuberculosis is one of the most clinically significant infectious agents. Especially during mass outbreaks, accurate identification and monitoring are required. The proportion of Beijing family members is very... -
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- Distribution of Virulence Genes in spa Types of Methicillin-resistant Staphylococcus aureus Isolated from Patients in Intensive Care Units
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Kim T, Yi J, Hong KH, Park JS, Kim EC
- KMID: 1096657
- Korean J Lab Med.
- 2011 Jan;31(1):30-36.
- doi: 10.3343/kjlm.2011.31.1.30
BACKGROUND: Various virulence factors and superantigens are encoded by mobile genetic elements. The relationship between clonal background and virulence factors differs in different geographic regions. We compared the distribution and... -
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- Application of Infrequent-Restriction-Site Amplification for Genotyping of Mycobacterium tuberculosis and non-tuberculous Mycobacterium
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Choi TY, Kang JO
- KMID: 1127247
- J Korean Med Sci.
- 2002 Oct;17(5):593-598.
- doi: 10.3346/jkms.2002.17.5.593
Infrequent restriction site amplification (IRS-PCR) is a method of amplifying DNA sequences, which flank an infrequent restriction site, and produces a strain-specific electrophoretic pattern. We studied the use of... -
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- Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
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Jang MH, Choi GE, Shin BM, Lee SH, Kim SR, Chang CL, Kim JM
- KMID: 1781693
- Korean J Lab Med.
- 2011 Oct;31(4):282-284.
- doi: 10.3343/kjlm.2011.31.4.282
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR... -
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- Epidemiological and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from the Ear Discharge of Outpatients with Chronic Otitis Media
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Yang JA, Kim JY, Yoon YK, Kim S, Park DW, Sohn JW, Sim HS, Kim MJ
- KMID: 1783057
- J Korean Med Sci.
- 2008 Oct;23(5):762-766.
- doi: 10.3346/jkms.2008.23.5.762
The origin of methicillin-resistant Staphylococcus aureus (MRSA) strains from otolaryngology outpatients has not been evaluated yet in Korea. We analyzed epidemiologic and genetic characteristics of MRSA isolates from the ear... -
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- A Study on Modes of Transmission and Role of Nasal Carriage to Subsequent Infection with Methicillin-Resistant Staphylococcus aureus in Medical ICU Using PFGE
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Kim S, Kim CK, Lee H, Peck KR, Kwon J, Lee JH, Lee NY, Kim YH, Kim OS, Yoon SW, Jin JH, Yang JW, Song JH
- KMID: 1558728
- Korean J Nosocomial Infect Control.
- 1998 Jun;3(1):1-10.
BACKGROUND: In Korea, methicillin-resistant Staphylococcus aureus (MRSA) is the most common nosocomial pathogen, which is particularly prevalent in ICU. We performed this study to investigate the modes of transmission of... -
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- Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based VITEK MS System for the Identification of Acinetobacter Species from Blood Cultures: Comparison with VITEK 2 and MicroScan Systems
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Lee SY, Shin JH, Kim SH, Shin MG, Suh SP, Ryang DW
- KMID: 2363149
- Ann Lab Med.
- 2015 Jan;35(1):62-68.
- doi: 10.3343/alm.2015.35.1.62
BACKGROUND: Acinetobacter species are the leading cause of bloodstream infection (BSI), but their correct identification is challenging. We evaluated the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based... -
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- Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism (RFLP)
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Singh SK, Verma R, Shah DH
- KMID: 1093628
- J Vet Sci.
- 2004 Dec;5(4):331-335.
Forty mycobacterial strains comprising clinical Indian isolates of Mycobacterium tuberculosis (28 field isolates +1H37 Rv) and Mycobacterium bovis (10 field isolates +1 AN5) were subjected to restriction fragment length polymorphism... -
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- Molecular Typing of Staphylococcus aureus Isolated from Blood on the Basis of Coagulase Gene Polymorphism and Toxin Genes
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Kim YK, Kim JS, Kim HS, Song W, Cho HC, Lee KM
- KMID: 854891
- Korean J Lab Med.
- 2008 Aug;28(4):286-292.
- doi: 10.3343/kjlm.2008.28.4.286
BACKGROUND: Coagulase is produced by all strains of Staphylococcus aureus. The 3' coding region of the coagulase (coa) gene contains varying numbers of 81 bp tandem repeats. S. aureus... -
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- Evaluation of the BD Phoenix Automated Microbiology System SMIC/ID-2 Panel for Antimicrobial Susceptibility Testing of Streptococcus pneumoniae
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Lee KK, Ryoo NH, Kim ST, Chae SL, Huh HJ
- KMID: 1096972
- Korean J Lab Med.
- 2009 Jun;29(3):212-217.
- doi: 10.3343/kjlm.2009.29.3.212
BACKGROUND: With the emergence of antimicrobial resistance among Streptococcus pneumoniae, a more accurate and automated antimicrobial susceptibility testing method is essential. We evaluated the BD Phoenix Automated Microbiology System (Becton... -
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- Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
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Huh HJ, Jang MA, Seo JY, Kim JY, Ki CS, Kim JW, Lee NY
- KMID: 2363151
- Ann Lab Med.
- 2015 Jan;35(1):76-81.
- doi: 10.3343/alm.2015.35.1.76
BACKGROUND: Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with... -
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