J Vet Sci.  2013 Sep;14(3):299-305. 10.4142/jvs.2013.14.3.299.

Dissimilarity of ccrAB gene sequences between methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus among bovine isolates in Korea

• Park YK
• Paik YH
• Yoon JW
• Fox LK
• Hwang SY
• Park YH

• KMID: 1705560
• DOI: http://doi.org/10.4142/jvs.2013.14.3.299

Abstract

The sequences of the ccrAB genes from bovine-, canine- and chicken-originating methicillin-resistant Staphylococcus (S.) epidermidis (MRSE) and bovine methicillin-resistant Staphylococcus (S.) aureus (MRSA) were compared to investigate the frequency of intra-species horizontal transfer of the staphylococcal cassette chromosome mec (SCCmec) complex. Nineteen MRSE strains were isolated from bovine milk, chickens, and dogs, and their genetic characteristics were investigated by multilocus sequence typing and SCCmec typing. Among the animal MRSE strains, the most frequent SCCmec type was type IV, which consisted of the type B mec complex and ccrAB type 2. The ccrA2 and ccrB2 genes were sequenced from the bovine, chicken and canine MRSE strains and compared with those of the bovine MRSA strains. The sequences generally clustered as MRSA and MRSE groups, regardless of the animal source. Additionally, no bovine MRSE sequence was associated with the bovine MRSA groups. Although most of the bovine MRSE and MRSA isolates possessed SCCmec type IV sequences, our results suggest that the intra-species gene transfer of the SCCmec complex between bovine S. aureus and bovine S. epidermidis strains is not a frequent event.

Keyword

ccrAB; methicillin-resistant Staphylococcus aureus; methicillin-resistant Staphylococcus epidermidis; SCCmec

MeSH Terms

Animals
Anti-Bacterial Agents/*pharmacology
Bacterial Proteins/*genetics/metabolism
Bacterial Typing Techniques/veterinary
Cattle
Cattle Diseases/epidemiology/metabolism
Chickens
Dog Diseases/epidemiology/metabolism
Dogs
*Drug Resistance, Bacterial
*Gene Transfer, Horizontal
Methicillin/*pharmacology
Methicillin-Resistant Staphylococcus aureus/genetics/isolation & purification
Milk/microbiology
Multilocus Sequence Typing/veterinary
Poultry Diseases/epidemiology/metabolism
Prevalence
Republic of Korea/epidemiology
Staphylococcal Infections/epidemiology/microbiology/*veterinary
Staphylococcus epidermidis/genetics/isolation & purification
Anti-Bacterial Agents
Bacterial Proteins
Methicillin

Figure

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  Fig. 1 Parsimony trees showing phylogenetic relationships among the ccrA2 (A) and ccrB2 (B) genes in MRSE and MRSA isolated from animal sources. Sequences from Staphylococcus (S.) aureus ccrA3 (A; GenBank accession no. AB037671) and S. aureus ccrB3 (B; GenBank accession no. AB037671) were used as outgroups.

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