Korean J Lab Med.  2011 Oct;31(4):282-284. 10.3343/kjlm.2011.31.4.282.

Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea

• Jang MH
• Choi GE
• Shin BM
• Lee SH
• Kim SR
• Chang CL
• Kim JM

• KMID: 1781693
• DOI: http://doi.org/10.3343/kjlm.2011.31.4.282

Abstract

Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab(TM) system (bioMerieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.

Keyword

Mycobacterium tuberculosis; Repetitive sequence-based polymerase chain reaction; DiversiLab Microbial Typing System

MeSH Terms

Automation
*Bacterial Typing Techniques
*Epidemiologic Methods
Genotype
Humans
Mycobacterium tuberculosis/*classification/genetics/isolation & purification
*Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reagent Kits, Diagnostic
Repetitive Sequences, Nucleic Acid
Republic of Korea/epidemiology
Tuberculosis/diagnosis/*epidemiology/microbiology

Figure

• 

  Fig. 1 Comparison of rep-PCR results with IS6110-RFLP patterns for 89 clinical Mycobacterium tuberculosis isolates. A scale for rep-PCR similarity (%) is shown at the bottom of the figure. IS6110-RFLP results are cited from reference 9.Abbreviations: RFLP, restriction fragment length polymorphism; NB, strains of non-Beijing family; B, strains of Beijing family; K, strains of K family.

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