- Comparison of L1 consensus primers with E6 type specific primers for detection of human papillomaviruses in paraffin sections of cervical neoplasia
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Park JS, Namkoong S, Han SK, Nha DJ, Lee HY, Kim SJ
- KMID: 1421953
- J Korean Med Sci.
- 1993 Feb;8(1):60-67.
- doi: 10.3346/jkms.1993.8.1.60
In our comparative study of L1 consensus primers with E6 type-specific primers for detection of human papillomavirus (HPVs) by polymerase chain reaction (PCR) in 35 cases of cervical neoplasia, the... -
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- Prenatal fetal sex determination from maternal peripheral blood using polymerase chain reaction
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Yang YH, Yoo HS, Kim IK
- KMID: 1066886
- Yonsei Med J.
- 1995 Aug;36(4):361-366.
- doi: 10.3349/ymj.1995.36.4.361
We have investigated the use of a nested polymerase chain reaction(PCR) assay with Y-specific sequence from the DYS 14 locus on the short arm of Y-chromosome for prenatal sex determination... -
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- Detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculosis or other pulmonary diseases by polymerase chain reaction
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Yoon KH, Cho SN, Lee TY, Cheon SH, Chang J, Kim SK, Chong Y, Chung DH, Lee WY, Kim JD
- KMID: 1120231
- Yonsei Med J.
- 1992 Sep;33(3):209-216.
- doi: 10.3349/ymj.1992.33.3.209
Polymerase chain reaction (PCR) using primers targeting the IS6110 repetitive sequence was employed to detect Mycobacterium tuberculosis in 228 samples from patients with tuberculosis or other pulmonary diseases and controls,... -
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- Application of PCR from the fine needle aspirates for the diagnosis of cervical tuberculous lymphadenitis
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Kim SS, Chung SM, Kim JN, Lee MA, Ha EH
- KMID: 644939
- J Korean Med Sci.
- 1996 Apr;11(2):127-132.
- doi: 10.3346/jkms.1996.11.2.127
Tuberculosis remains a major public health problem worldwide. A definitive and accurate diagnosis of tuberculosis in cervical lymphadenopathy is important because satisfactory results can be achieved with chemotherapy alone, obviating... -
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- PCR in diagnosis of pneumocystosis of rats
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Hong ST
- KMID: 1715355
- Korean J Parasitol.
- 1996 Sep;34(3):191-196.
- doi: 10.3347/kjp.1996.34.3.191
Polymerase chain reaction (PCR) is a powerful technique to detect scanty amount of DNA from living organisms. The present study intended to develop specific primers for PCR diagnosis of pneumocystosis... -
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- Evaluation of Direct Immunofluorescence Test with PCR for Detection of Novel Influenza A (H1N1) Virus during 2009 Pandemic
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Lee JH, Shin SR, Cho JH
- KMID: 1728003
- Yonsei Med J.
- 2011 Jul;52(4):680-682.
- doi: 10.3349/ymj.2011.52.4.680
During the 2009 novel influenza (H1N1) pandemic, the sensitivity of direct immunofluorescence assay (DFA) for H1N1 infection was 62% (266/429) of that of the polymerase chain reaction (PCR) test. The... -
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- Rapid Prenatal Diagnosis of Trisomy 21 by Real-time Quantitative Polymerase Chain Reaction with Amplification of Small Tandem Repeats and S100B in Chromosome 21
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Yang YH, Nam MS, Yang ES
- KMID: 1734043
- Yonsei Med J.
- 2005 Apr;46(2):193-197.
- doi: 10.3349/ymj.2005.46.2.193
Trisomy 21 (Down syndrome) is the most common congenital anomaly, and it occurs in one out of 700-1000 births. Current techniques such as amniocentesis and chorionic villi sampling (CVS) require... -
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- Detection and Typing of HSV-1, HSV-2, CMV and EBV by Quadruplex PCR
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Shin CH, Park GS, Hong KM, Paik MK
- KMID: 1091040
- Yonsei Med J.
- 2003 Dec;44(6):1001-1007.
- doi: 10.3349/ymj.2003.44.6.1001
The development of a multiplex polymerase chain reaction (PCR) method for rapid and accurate detection and typing of herpes simplex virus type 1 (HSV-1), and type-2 (HSV-2), cytomegalovirus (CMV) and... -
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- A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae
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Suh B, Chun JK, Yong D, Lee YS, Jeong SH, Yang WI, Kim DS
- KMID: 1096816
- Korean J Lab Med.
- 2010 Feb;30(1):34-37.
- doi: 10.3343/kjlm.2010.30.1.34
We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the... -
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- Detection of Mycobacterial DNA Using Nested Polymerase Chain Reaction of Pleural Biopsy Specimens: Compared to Pathologic Findings
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Yum HK, Choi SJ
- KMID: 1106141
- Korean J Intern Med.
- 2003 Jun;18(2):89-93.
BACKGROUND: Although there are many methods including AFB smear and culture, and the analysis of pleural fluid in the etiological diagnosis of pleural effusion, it is sometimes difficult to confirm... -
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- A PCR-RFLP method for the detection of activated H-ras oncogene with a point mutation at codon 12 and 61
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Hong SJ, Lee T, Park YS, Lee KO, Chung BH, Lee SH
- KMID: 1728886
- Yonsei Med J.
- 1996 Dec;37(6):371-379.
- doi: 10.3349/ymj.1996.37.6.371
To investigate the incidence of the H-ras gene activation in bladder tumor and the feasibility of using urinary washout samples for screening, a series of 33 human bladder tumors and... -
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- Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea
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Jang MH, Choi GE, Shin BM, Lee SH, Kim SR, Chang CL, Kim JM
- KMID: 1781693
- Korean J Lab Med.
- 2011 Oct;31(4):282-284.
- doi: 10.3343/kjlm.2011.31.4.282
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR... -
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- Methicillin-resistant Staphylococcus aureus in Nasal Surveillance Swabs at an Intensive Care Unit: An Evaluation of the LightCycler MRSA Advanced Test
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Huh HJ, Kim ES, Chae SL
- KMID: 2434481
- Ann Lab Med.
- 2012 Nov;32(6):407-412.
BACKGROUND: We compared the LightCycler MRSA advanced test (Roche Diagnostics, Germany) with enrichment culture methods to evaluate the relative diagnostic performance of the LightCycler MRSA advanced test for active surveillance... -
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- Discrepancy in Genotyping of Apolipoprotein E between Allele-Specific PCR and Fluorescence Resonance Energy Transfer or Sequencing
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Park CH, Lee ST, Ki CS, Kim JW
- KMID: 1781635
- Korean J Lab Med.
- 2010 Jun;30(3):325-328.
- doi: 10.3343/kjlm.2010.30.3.325
The human apolipoprotein E (APOE) gene contains several single-nucleotide polymorphisms (SNPs) that are distributed across the gene. The genotype of the APOE gene has important implications as a risk factor... -
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- Epidemiological Aspects of Pertussis among Adults and Adolescents in a Korean Outpatient Setting: A Multicenter, PCR-Based Study
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Park S, Lee SH, Seo KH, Shin KC, Park YB, Lee MG, Yoo KH, Kim HJ, Park JS, Cho JH, Ko Y, Lee SK, Cheon KT, Kim DI, Ha JW, Lee JM, Suhr JW, Jeong EH, Jung KS
- KMID: 1794601
- J Korean Med Sci.
- 2014 Sep;29(9):1232-1239.
- doi: 10.3346/jkms.2014.29.9.1232
Epidemiological data of Bordetella pertussis infection among adolescents and adults are limited in Korea. Patients (> or = 11 yr of age) with a bothersome cough for less than 30... -
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- Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey
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Inat G, Siriken B
- KMID: 1072181
- J Vet Sci.
- 2010 Dec;11(4):321-326.
- doi: 10.4142/jvs.2010.11.4.321
This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected... -
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- An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting
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Hawash Y, Ghonaim MM, Al-Shehri
- KMID: 2374010
- Korean J Parasitol.
- 2016 Feb;54(1):1-8.
- doi: 10.3347/kjp.2016.54.1.1
Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round... -
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- Evaluation of the Seeplex(R) Meningitis ACE Detection Kit for the Detection of 12 Common Bacterial and Viral Pathogens of Acute Meningitis
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Shin SY, Kwon KC, Park JW, Kim JM, Shin SY, Koo SH
- KMID: 1781437
- Ann Lab Med.
- 2012 Jan;32(1):44-49.
- doi: 10.3343/alm.2012.32.1.44
BACKGROUND: Bacterial meningitis is an infectious disease with high rates of mortality and high frequency of severe sequelae. Early identification of causative bacterial and viral pathogens is important for prompt... -
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- Detection of Vancomycin-resistant Enterococci using Multiplex Real-time PCR Assay and Melting Curve Analysis
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Cha CH, An HK, Kim JU
- KMID: 1096799
- Korean J Lab Med.
- 2010 Apr;30(2):138-146.
- doi: 10.3343/kjlm.2010.30.2.138
BACKGROUND: We developed and evaluated the utility of a multiplex real-time PCR assay that uses melting curve analysis and allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. METHODS: The... -
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- Evaluation of the Efficacies of Rapid Antigen Test, Multiplex PCR, and Real-time PCR for the Detection of a Novel Influenza A (H1N1) Virus
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Hwang Y, Kim K, Lee M
- KMID: 1096800
- Korean J Lab Med.
- 2010 Apr;30(2):147-152.
- doi: 10.3343/kjlm.2010.30.2.147
BACKGROUND: In April 2009, a novel influenza A (H1N1) virus was detected in the US, and at the time of conducting this study, H1N1 infection had reached pandemic proportions. In... -
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