Ann Lab Med.  2016 Jan;36(1):9-14. 10.3343/alm.2016.36.1.9.

Epidemiological Study of Erythromycin-Resistant Streptococcus pyogenes From Korea and Japan by emm Genotyping and Multilocus Sequence Typing

• Takahashi
• Arai K
• Lee DH
• Koh EH
• Yoshida H
• Yano H
• Kaku M
• Kim S

• KMID: 2373491
• DOI: http://doi.org/10.3343/alm.2016.36.1.9

Abstract

BACKGROUND
We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST).
METHODS
Clinical isolates of GAS had been collected from 1992 to 2012 in Korea and from 2004 to 2009 in Japan. EM resistance was determined by the microdilution method, and resistance genotypes were assessed by PCR. The emm genotyping and MLST were performed by DNA sequencing.
RESULTS
The emm genotypes and sequence types (STs) were concordant in 143 (85.1%) of 168 EM-resistant GAS strains from Korea. ST36/emm12 (35.1%), ST52/emm28 (22.6%), and ST49/emm75 (16.1%) were the most common types. Most of the ST36 (93.9%) and ST52 (95.8%) strains harbored erm(B), whereas strains ST49, ST42, and ST15 contained mef(A). The concordance between emm genotypes and STs was 41 (93.2%) among 44 EM-resistant GAS strains from Japan. ST36/emm12 (34.1%), ST49/emm75 (18.2%), and ST28/emm1 (15.9%) were the major types. ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A). The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively.
CONCLUSIONS
The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan. Genotype erm(B) was predominant in Korea, whereas mef(A) was frequent in Japan. There were differences between Korea and Japan regarding the frequencies of emm genotypes, STs, and EM resistance genes among the EM-resistant GAS.

Keyword

Streptococcus pyogenes; Genotypes; Sequence types; Erythromycin; Resistance

MeSH Terms

Anti-Bacterial Agents/*pharmacology/therapeutic use
Bacterial Proteins/*genetics
Bacterial Typing Techniques
*Drug Resistance, Bacterial
Epidemiologic Studies
Erythromycin/*pharmacology/therapeutic use
Genotype
Humans
Japan/epidemiology
Microbial Sensitivity Tests
Multilocus Sequence Typing
Republic of Korea/epidemiology
Streptococcal Infections/drug therapy/*microbiology
Streptococcus pyogenes/drug effects/*genetics/isolation & purification
Anti-Bacterial Agents
Bacterial Proteins
Erythromycin

Figure

• 

  Fig. 1 Prevalence of sequence types (STs) by different periods in Korea (A) and in Japan (B).

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