Korean J Lab Med.  2009 Jun;29(3):212-217. 10.3343/kjlm.2009.29.3.212.

Evaluation of the BD Phoenix Automated Microbiology System SMIC/ID-2 Panel for Antimicrobial Susceptibility Testing of Streptococcus pneumoniae

• Lee KK
• Ryoo NH
• Kim ST
• Chae SL
• Huh HJ

• KMID: 1096972
• DOI: http://doi.org/10.3343/kjlm.2009.29.3.212

Abstract

BACKGROUND: With the emergence of antimicrobial resistance among Streptococcus pneumoniae, a more accurate and automated antimicrobial susceptibility testing method is essential. We evaluated the BD Phoenix Automated Microbiology System (Becton Dickinson Diagnostic Systems, USA) SMIC/ID-2 panel for antimicrobial susceptibility testing of S. pneumoniae.
METHODS
A total of 113 clinical strains of S. pneumoniae (88 penicillin susceptible strains, 8 intermediate strains, and 17 resistant strains by 2008 CLSI criteria) were tested. Minimum inhibitory concentrations (MICs) for penicillin, cefotaxime, clindamycin, erythromycin, levofloxacin, trimethoprim/ sulfamethoxazole, tetracycline, and vancomycin were determined by Etest (AB Biodisk, Sweden) and Phoenix System. The results obtained by Phoenix system were compared to those obtained by Etest.
RESULTS
The overall essential agreement of MICs (within one dilution of MICs) defined by the Phoenix and Etest was 92.3%. Neither very major errors nor major errors were produced, and minor errors were 6.5%. Minor errors were frequently observed in susceptibility testings for penicillin (22.1%), cefotaxime (12.4%), and trimethoprim/sulfamethoxazole (11.5%).
CONCLUSIONS
The Phoenix SMIC/ID-2 panel provided a simple and rapid susceptibility testing for S. pneumoniae, and the results were in a good agreement with those of Etest. The Phoenix system appears to be an effective automated system in clinical microbiology laboratories.

Keyword

Streptococcus pneumoniae; BD Phoenix; Antimicrobial susceptibility test; Etest

MeSH Terms

Anti-Bacterial Agents/pharmacology
Bacterial Typing Techniques/instrumentation/methods
Drug Resistance, Bacterial
Microbial Sensitivity Tests/*methods
Reagent Kits, Diagnostic
Streptococcus pneumoniae/*drug effects/growth & development/isolation & purification

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