- Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR
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Kim MN, Ko YJ, Seong MW, Kim JS, Shin BM, Sung H
- KMID: 2373575
- Ann Lab Med.
- 2016 Sep;36(5):450-456.
- doi: 10.3343/alm.2016.36.5.450
BACKGROUND: During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in... -
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- Comparison of Quantitation of Cytomegalovirus DNA by Real-Time PCR in Whole Blood with the Cytomegalovirus Antigenemia Assay
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Kwon S, Jung BK, Ko SY, Lee CK, Cho Y
- KMID: 2363155
- Ann Lab Med.
- 2015 Jan;35(1):99-104.
- doi: 10.3343/alm.2015.35.1.99
BACKGROUND: Quantitation of cytomegalovirus (CMV) DNA using real-time PCR has been utilized for monitoring CMV infection. However, the CMV antigenemia assay is still the 'gold standard' assay. There are only... -
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- Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood
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Park JE, Kim JY, Yun SA, Lee MK, Huh HJ, Kim JW, Ki CS
- KMID: 2373601
- Ann Lab Med.
- 2016 Nov;36(6):603-606.
- doi: 10.3343/alm.2016.36.6.603
Standardized cytomegalovirus (CMV) DNA quantification is important for managing CMV disease. We evaluated the performance of the Real-Q CMV Quantification Kit (Real-Q assay; BioSewoom, Korea) using whole blood (WB), with... -
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- The Combination of Real-Time PCR and HPLC for the Identification of Non-Tuberculous Mycobacteria
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Park JS, Choi JI, Lim JH, Ahn JJ, Jegal Y, Seo KW, Ra SW, Jeon JB, Lee SH, Kim SR, Jeong J
- KMID: 1781340
- Ann Lab Med.
- 2013 Sep;33(5):349-352.
- doi: 10.3343/alm.2013.33.5.349
We used HPLC and AdvanSure real-time PCR (LG Life Sciences, Korea) to retrospectively analyze non-tuberculous mycobacteria (NTM) in 133 clinical specimens. The specimens were culture-positive for NTM and the HPLC... -
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- Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens
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Choi YJ, Kim HJ, Shin HB, Nam HS, Lee SH, Park JS, Park KS, Baek KA
- KMID: 1380084
- Ann Lab Med.
- 2012 Jul;32(4):257-263.
- doi: 10.3343/alm.2012.32.4.257
BACKGROUND: A peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR(TM) TB/NTM detection kit; PANAGENE, Korea) assay has been recently developed for the simultaneous detection of Mycobacterium tuberculosis complex (MTBC) and... -
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- Evaluation of a Real-Time PCR Assay for Simultaneous Detection of Kingella kingae and Staphylococcus aureus from Synovial Fluid in Suspected Septic Arthritis
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Haldar M, Butler M, Quinn CD, Stratton C, Tang YW, Burnham CA
- KMID: 1791940
- Ann Lab Med.
- 2014 Jul;34(4):313-316.
- doi: 10.3343/alm.2014.34.4.313
Direct plating of synovial fluid (SF) on agar-based media often fails to identify pathogens in septic arthritis (SA). We developed a PCR assay for the simultaneous detection of Kingella kingae... -
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- Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
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Kim YJ, Lee SM, Park BK, Kim SS, Yi J, Kim HH, Lee EY, Chang CL
- KMID: 1791921
- Ann Lab Med.
- 2014 May;34(3):203-209.
- doi: 10.3343/alm.2014.34.3.203
BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether... -
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- Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods
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Lee H, Park KG, Lee G, Park J, Park YG, Park YJ
- KMID: 1781360
- Ann Lab Med.
- 2014 Jan;34(1):51-55.
- doi: 10.3343/alm.2014.34.1.51
Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM... -
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- Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
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Huh HJ, Jang MA, Seo JY, Kim JY, Ki CS, Kim JW, Lee NY
- KMID: 2363151
- Ann Lab Med.
- 2015 Jan;35(1):76-81.
- doi: 10.3343/alm.2015.35.1.76
BACKGROUND: Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with... -
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- Real-Time Nucleic Acid Sequence-Based Amplification to Predict the Clinical Outcome of Invasive Aspergillosis
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Kim SH, Park C, Kwon EY, Shin NY, Kwon JC, Park SH, Choi SM, Lee DG, Choi JH, Yoo JH
- KMID: 1792972
- J Korean Med Sci.
- 2012 Jan;27(1):10-15.
- doi: 10.3346/jkms.2012.27.1.10
Monitoring the response to therapy for invasive aspergillosis (IA) is essential for the management of patients with hematologic diseases. We evaluated the correlation between the outcome of real-time nucleic acid... -
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- Evaluation of PCR-Reverse Blot Hybridization Assay, REBA Sepsis-ID Test, for Simultaneous Identification of Bacterial Pathogens and mecA and van Genes from Blood Culture Bottles
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Park SD, Lee G, Wang HY, Park M, Kim S, Kim H, Kim J, Kim YK, Kim HY, Lee H, Uh Y, Kim JB
- KMID: 2129570
- Ann Lab Med.
- 2014 Nov;34(6):446-455.
- doi: 10.3343/alm.2014.34.6.446
BACKGROUND: The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia... -
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- Comparative Evaluation of Three Chromogenic Media Combined with Broth Enrichment and the Real-Time PCR-Based Xpert MRSA Assay for Screening of Methicillin-Resistant Staphylococcus aureus in Nasal Swabs
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Lee S, Park YJ, Park KG, Jekarl DW, Chae H, Yoo JK, Seo SW, Choi JE, Lim JH, Heo SM, Seo JH
- KMID: 1707232
- Ann Lab Med.
- 2013 Jul;33(4):255-260.
- doi: 10.3343/alm.2013.33.4.255
BACKGROUND: We evaluated the performance of three chromogenic media (Brilliance agar I [Oxoid, UK], Brilliance agar II [Oxoid], and ChromID MRSA [Biomerieux, France]) combined with broth enrichment and the Xpert... -
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- Clinical Validation of AdvanSure GenoBlot Assay as Primary Screening and Test of Cure for Human Papillomavirus Infection
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Kahng J, Oh EJ, Lee HN, Lee DW, Kim Y
- KMID: 1791905
- Ann Lab Med.
- 2014 Mar;34(2):127-133.
- doi: 10.3343/alm.2014.34.2.127
BACKGROUND: Clinical specificity and sensitivity are essential factors in the adoption of a human papillomavirus (HPV) test as a primary screening tool and test of cure after treatment of cervical... -
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