Exp Mol Med.  2001 Sep;33(3):169-173.

Cloning of BNIP3h, a member of proapoptotic BNIP3 family genes

• Farooq M
• Kim YH
• Im SU
• Chung EJ
• Hwang SY
• Sohn MY
• Kim MK
• Kim JC

Abstract

Apoptosis is regulated by interaction of antiapoptotic Bcl-2 family proteins with various proapoptotic proteins, several of which are also members of the Bcl-2 family. BNIP3 (formerly NIP3) is a proapoptotic mitochondrial protein classified in the Bcl-2 family based on limited sequence homology-3 (BH3) domain and COOH-terminal transmembrane domain. Sequence comparison of BNIP3 has indicated that there are several BNIP3 human homologs of this protein, like BNIP3L, Nix and BNIP3. We have cloned a new member of BNIP3 family from the cDNA library prepared from human dermal papilla cells and designated as BNIP3h. BNIP3h shows substantial homology with other BNIP3 family proteins. BNIP3h induced apoptosis from 24 hours after transfection in MCF7 cell lines and its apoptosis inducing activity is extended until 72 hours after transfection.

Keyword

Apoptosis; BNIP3 family proteins; BNIP3h

MeSH Terms

Amino Acid Sequence
Apoptosis/*physiology
Base Sequence
Cells, Cultured
Cloning, Molecular
Dermis/chemistry/cytology
Human
Membrane Proteins/chemistry/*genetics/metabolism
Mitochondria/chemistry
Molecular Sequence Data
Multigene Family
Sequence Alignment
Tissue Distribution
Transfection
Tumor Cells, Cultured