Korean J Intern Med.  1998 Jul;13(2):104-109.

Antigenic diversity and serotypes of Helicobacter pylori associated with peptic ulcer diseases

• Park SM
• Hong SI
• Jung HY
• Yang SK
• Kim HR
• Min YI
• Hong WS

Abstract

OBJECTIVES
Clinical presentation of Helicobacter pylori (H. pylori) infection has marked variation mainly due to the strain diversity and host susceptibility. Although H. pylori is identified as a major risk factor for gastric and duodenal ulcers, the ulcerogenic or pathogenic strain has not been documented yet. The
objective
of this study was to investigate antigenic types of the ulcerogenic strain of H. pylori. METHODS: The sera of 64 patients were tested by Western blot using Helicoblot 2.0 for six major anti-H. pylori antibodies, together with CLO test and histological examination of gastric biopsy tissues. Thirty-five, nine and 20 patients had duodenal ulcer, gastric ulcer and chronic active gastritis, respectively. The antigenic types of H. pylori were analyzed in 54 patients with positive H. pylori infection. In this study, H. pylori was divided into four serotypes according to the presence and absence of CagA and VagA: type I; CagA (+) and VacA(+), type Ia: CagA (+) and VacA(-), type Ib: CagA(-) and VacA(+), and type II: CagA(-) and VacA(-). RESULTS: There was no difference in the number of bands for six antigens: 3.2 +/- 1.4, 3.0 +/- 1.2 and 3.1 +/- 1.4 in 35 duodenal ulcer, 7 gastric ulcer and 12 chronic gastritis, respectively. The band with 119 kDa was 90.7%, which was the most common band with the order of 35, 30, 26.5, 89 and 19.5 kDa. Type I, la and Ib were positive in 22.2, 42.6 and 27.8%, respectively, which were significantly higher than type II (p < 0.05). There was no difference in the positive rates of four urease subtypes between the four serotypes.

MeSH Terms

Adult
Aged
Antigens, Bacterial/classification*
Antigens, Bacterial/analysis
Blotting, Western
Chronic Disease
Comparative Study
Duodenal Ulcer/pathology
Duodenal Ulcer/microbiology*
Duodenal Ulcer/immunology
Gastric Mucosa/pathology
Gastric Mucosa/microbiology
Gastritis/pathology
Gastritis/microbiology
Gastritis/immunology
Helicobacter Infections/immunology*
Helicobacter pylori/immunology*
Human
Middle Age
Serotyping
Stomach Ulcer/pathology
Stomach Ulcer/microbiology*
Stomach Ulcer/immunology
Substances: Antigens, Bacterial