Exp Mol Med.  2000 Dec;32(4):179-186.

Atrial natriuretic peptide induces rat peritoneal mast cell activation by cGMP-independent and calcium uptake-dependent mechanism

• Chai OH
• Lee YH
• Han EH
• Kim HT
• Lee MS
• Song CH

Abstract

Atrial natriuretic peptide (ANP), a 28 amino acid basic polypeptide, is known to induce histamine release from human and rat mast cells in vitro and cause a wheel formation in rat skin. However, cellular events associated with histamine release are not clearly understood. In this study, we have examined the calcium flux and cGMP formation associated with histamine release in the ANP-treated mast cells. ANP, in vitro, induced mast cell degranulation and histamine release in a dose-dependent manner. ANP also induced an enhanced calcium uptake into cells and increased the cellular level of cGMP in mast cells. A high level of calcium in the media caused an inhibition of ANP-dependent histamine release but enhanced the level of intracellular cGMP of mast cells. ANP inducing a dose-dependent increase in vascular permeability of rat skin was confirmed by the extravasation of the circulating Evans blue. The results indicate ANP induced the histamine release and an increase in vascular permeability through mast cell degranulation in cGMP-independent and calcium uptake-dependent manner.

Keyword

ANP; mast cell; histamine; calcium; cGMP

MeSH Terms

Animal
Atrial Natriuretic Factor/*pharmacology
Biological Transport
Calcium/*metabolism
Capillary Permeability
Cell Degranulation
Cyclic GMP/*metabolism
Dose-Response Relationship, Drug
*Histamine Release
Mast Cells/*drug effects
Peritoneal Cavity/cytology
Rats