J Korean Med Sci.  1998 Oct;13(5):507-512. 10.3346/jkms.1998.13.5.507.

p21 expression and mutation in gastric carcinoma: analysis by immunohistochemistry and PCR-SSCP

• Park YE
• Choi KC
• Choi YH

• KMID: 645210
• DOI: http://doi.org/10.3346/jkms.1998.13.5.507

Abstract

p21 protein has been reported to be a critical downstream effector of p53 and a potent inhibitor of cyclin-dependent kinases. Thus, the p21 gene is thought to play a central role in tumor suppression. In this study we investigated p21 protein expression and mutation in gastric adenocarcinoma. A total of 76 primary gastric carcinoma specimens were immunohistochemically stained for p21 protein expression and evaluated the correlations between p21 expression and clinicopathologic features. In a proportion of them (20 cases), we also analyzed the possible presence of p21 gene mutations using PCR-SSCP method. Fourty seven out of 76 cases (61.8%) were p21-negative, and the remaining twenty nine cases (38.2%) were p21 -positive on immunostains. There was a correlation between the expression of p21, and the depth of tumor invasion and lymph node metastasis (p<0.05). No mutation of the p21 gene was detected in all of 20 tumor tissues. These results suggest that the status of p21 expression may have prognostic value in gastric adenocarcinoma.

MeSH Terms

Adenocarcinoma/pathology
Adenocarcinoma/metabolism*
Adult
Aged
Cyclins/genetics*
Female
Gene Expression
Human
Immunoenzyme Techniques
Male
Middle Age
Mutation*
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Sequence Analysis, DNA
Staining and Labeling
Stomach Neoplasms/pathology
Stomach Neoplasms/metabolism*