Korean J Gastroenterol.
1997 May;29(5):601-609.
Detection of p53 Mutation in Gastric Cancer by Nonradioactive SSCP(mini-SSCP)
Abstract
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BACKGROUND/AIMS: p53 mutation was reported in many studies, but conventional PCR-SSCP protocol using radiolabeled PCR primers or nucleotides to generate a radioactive PCR products is cumbersome to handle. With the aim of a rapid and safe method for single-strand conformation polymorphism(SSCP) analysis of PCR products, we used Mini-SSCP method to detect the mutation of p53 gene of gastric cancer.
METHODS
In 27 pair of gastric cancer and normal tissues, PCR-SSCP was done with Mini-SSCP, and then Silver sequencing was done.
RESULTS
The p53 gene mutation of gastric cancer was found in 10 of 27(37%). Mutation of exon 4, 5 were 2, 5 cases and tnutation of exon 6, 7 mutation were 0, 3 cases respectively. Mutation rate was similar with other reports. P53 mutation was not correlated with stage, lymph node involvement and histological differentiation.
CONCLUSIONS
Mini-SSCP method, modification of Cold-SSCP, greately increased our accessibility to detect polymorphisms with easiness and safeness. So, it is a useful method to detect genetic mutation.
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