Tissue Eng Regen Med.  2024 Aug;21(6):915-927. 10.1007/s13770-024-00643-3.

Adipose-Derived Mesenchymal Stem Cells and Their Derived Epidermal Progenitor Cells Conditioned Media Ameliorate Skin Aging in Rats

• Badr OI
• Anter A
• Magdy I
• Chukueggu M
• Khorshid M
• Darwish M
• Farrag M
• Elsayed M
• Amr Y
• Amgad Y
• Mahmoud T
• Kamal MM

• KMID: 2558471
• DOI: http://doi.org/10.1007/s13770-024-00643-3

Abstract

BACKGROUND
Skin alterations are among the most prominent signs of aging, and they arise from both intrinsic and extrinsic factors that interact and mutually influence one another. The use of D-galactose as an aging model in animals has been widely employed in anti-aging research. Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are particularly promising for skin anti-aging therapy due to their capacity for effective re-epithelization and secretion of various growth factors essential for skin regeneration. Accordingly, we aimed to examine the potential utility of Ad-MSCs as a therapy for skin anti-aging.
METHODS
In this study, we isolated and characterized adipose-derived mesenchymal stem cells (Ad-MSCs) from the epididymal fat of male Sprague Dawley rats. We assessed the in vitro differentiation of Ad-MSCs into epidermal progenitor cells (EPCs) using ascorbic acid and hydrocoritsone. Additionally, we induced skin aging in female Sprague Dawley rats via daily intradermal injection of D-galactose over a period of 8 weeks. Then we evaluated the therapeutic potential of intradermal transplantation of Ad-MSCs and conditioned media (CM) derived from differentiated EPCs in the D-galactose-induced aging rats. Morphological assessments, antioxidant assays, and histopathological examinations were performed to investigate the effects of the treatments.
RESULTS
Our findings revealed the significant capability of Ad-MSCs to differentiate into EPCs. Notably, compared to the group that received CM treatment, the Ad-MSCs-treated group exhibited a marked improvement in morphological appearance, antioxidant levels and histological features.
CONCLUSIONS
These results underscore the effectiveness of Ad-MSCs in restoring skin aging as a potential therapy for skin aging.

Keyword

Skin aging; D-galactose; Adipose tissue; Mesenchymal stem cells; Epidermal progenitor cells; Regenerative medicine