Lab Anim Res.  2023 Dec;39(4):356-370. 10.1186/s42826-023-00178-z.

Promising improvement in infected Wound Healing in Type two Diabetic rats by Combined effects of conditioned medium of human adipose‑derived stem cells plus Photobiomodulation

• Sohrabi K
• Ahmadi H
• Amini A
• Ahrabi B
• Mostafavinia A
• Omidi H
• Mirzaei M
• Fathabady F
• Fridoni M
• Rahmannia M
• Chien S
• Bayat M

• KMID: 2554157
• DOI: http://doi.org/10.1186/s42826-023-00178-z

Abstract

Background
We aimed to examine the accompanying and solo impacts of conditioned medium of human adiposederived stem cells (h-ASC-COM) and photobiomodulation (PBM) on the maturation stage of an ischemic infected delayed-healing wound model (IIDHWM) of rats with type 2 diabetes (TIIDM).
Results
Outcomes of the wound closure ratio (WCR) results, tensiometrical microbiological, and stereological assessment followed almost identical patterns. While the outcomes of h-ASC-COM + PBM, PBM only, and h-ASCCOM only regimes were significantly better for all evaluated methods than those of group 1(all, p < 0.001), PBM alone and h-ASC-COM + PBM therapy achieved superior results than h-ASC-COM only (ranged from p = 0.05 to p < 0.001). In terms of tensiometrical and stereological examinations, the results of h-ASC-COM + PBM experienced better results than the PBM only (all, p < 0.001).
Conclusions
h-ASC-COM + PBM, PBM, and h-ASC-COM cures expressively accelerated the maturation stage in the wound healing process of IIDHWM with MRSA in TIIDM rats by diminishing the inflammatory reaction, and the microbial flora of MRSA; and increasing wound strength, WCR, number of fibroblasts, and new blood vessels. While the h-ASC-COM + PBM and PBM were more suitable than the effect of h-ASC-COM, the results of h-ASCCOM + PBM were superior to PBM only.

Keyword

Diabetes mellitus; Diabetic foot ulcers; Human adipose-derived stem cell; Conditioned medium; Photobiomodulation; Microbial flora; Stereology; Rat