J Vet Sci.
2023 Mar;24(2):e24. 10.4142/jvs.22204.
Antioxidant effect of ergothioneine on in vitro maturation of porcine oocytes
- Affiliations
-
- 1Veterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju 28644, Korea
- 2Institute of Stem Cell & Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea
- 3Graduate School of Veterinary Biosecurity and Protection, Chungbuk National University, Cheongju 28644, Korea
- 4College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea
- KMID: 2541341
- DOI: http://doi.org/10.4142/jvs.22204
Abstract
- Background
Ergothioneine (EGT) is a natural amino acid derivative in various animal organs and is a bioactive compound recognized as a food and medicine.
Objectives
This study examined the effects of EGT supplementation during the in vitro maturation (IVM) period on porcine oocyte maturation and subsequent embryonic development competence after in vitro fertilization (IVF).
Methods
Each EGT concentration (0, 10, 50, and 100 µM) was supplemented in the maturation medium during IVM. After IVM, nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels of oocytes were investigated. In addition, the genes related to cumulus function and antioxidant pathways in oocytes or cumulus cells were investigated. Finally, this study examined whether EGT could affect embryonic development after IVF.
Results
After IVM, the EGT supplementation group showed significantly higher intracellular GSH levels and significantly lower intracellular ROS levels than the control group. Moreover, the expression levels of hyaluronan synthase 2 and Connexin 43 were significantly higher in the 10 µM EGT group than in the control group. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and NAD(P)H quinone dehydrogenase 1 (NQO1) were significantly higher in the oocytes of the 10 µM EGT group than in the control group. In the assessment of subsequent embryonic development after IVF, the 10 µM EGT treatment group improved the cleavage and blastocyst rate significantly than the control group.
Conclusions
Supplementation of EGT improved oocyte maturation and embryonic development by reducing oxidative stress in IVM oocytes.
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