Tissue Eng Regen Med.
2022 Jun;19(3):491-503. 10.1007/s13770-021-00429-x.
The Biocompatibility of Multi-Source Stem Cells and GelatinCarboxymethyl Chitosan-Sodium Alginate Hybrid Biomaterials
- Affiliations
-
- 1Clinical Medical College, Jining Medical University, Jining 272067, Shandong, China
- 2Medical Research Center, Affiliated Hospital of Jining Medical University, Jining 272067, Shandong, China
- 3Department of Biochemistry, Jining Medical University, Jining, Shandong 272067, People’s Republic of China
- 4Collaborative Innovation Center, Jining Medical University, Jining, Shandong 272067, People’s Republic of China
- 5Basic Medicine College, Jining Medical University, Jining, Shandong 272067, People’s Republic of China
- 6Department of Urology, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, Shandong 272100, People’s Republic of China
- KMID: 2530143
- DOI: http://doi.org/10.1007/s13770-021-00429-x
Abstract
- BACKGROUND
Nowadays, biological tissue engineering is a growing field of research. Biocompatibility is a key indicator for measuring tissue engineering biomaterials, which is of great significance for the replacement and repair of damaged tissues.
METHODS
In this study, using gelatin, carboxymethyl chitosan, and sodium alginate, a tissue engineering material scaffold that can carry cells was successfully prepared. The material was characterized by Fourier transforms infrared spectroscopy. In addition, the prepared scaffolds have physicochemical properties, such as swelling ratio, biodegradability. we observed the biocompatibility of the hydrogel to different adult stem cells (BMSCs and ADSCs) in vivo and in vitro. Adult stem cells were planted on gelatin-carboxymethyl chitosan-sodium alginate (Gel/SA/CMCS) hydrogels for 7 days in vitro, and the survival of stem cells in vitro was observed by live/died staining. Gel/SA/CMCS hydrogels loaded with stem cells were subcutaneously transplanted into nude mice for 14 days of in vivo culture observation. The survival of adult stem cells was observed by staining for stem cell surface markers (CD29, CD90) and Ki67.
RESULTS
The scaffolds had a microporous structure with an appropriate pore size (about 80 lm). Live/died staining showed that adult stem cells could stably survive in Gel/SA/CMCS hydrogels for at least 7 days. After 14 days of culture in nude mice, Ki67 staining showed that the stem cells supported by Gel/SA/CMCS hydrogel still had high proliferation activity.
CONCLUSION
Gel/SA/CMCSs hydrogel has a stable interpenetrating porous structure, suitable swelling performance and degradation rate, can promote and support the survival of adult stem cells in vivo and in vitro, and has good biocompatibility. Therefore, Gel/SA/CMCS hydrogel is a strong candidate for biological tissue engineering materials.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Preadipocyte Culture in Chitosan-Alginate Gel
- Suppression of Fibrotic Reactions of Chitosan-Alginate Microcapsules Containing Porcine Islets by Dexamethasone Surface Coating
- Evaluation of the Potential Effects of Retinol and Alginate/Gelatin-Based Scaffolds on Differentiation Capacity of Mouse Mesenchymal Stem Cells (MSCs) into Retinal Cells
- Effect of oral ingestion of chitosan and alginate on the removal of orally ingested radiostrontium(Sr) in mice
- Chitosan-Poly(Vinyl Alcohol) Nanofibers by Free Surface Electrospinning for Tissue Engineering Applications
