Nat Prod Sci.  2021 Dec;27(4):228-233. 10.20307/nps.2021.27.4.228.

Chemical Constituents from Agrimonia pilosa with Inhibitory Activity against Interleukin 1β Production via NLRP3 and NLRC4 Inflammasomes

• Nhoek P
• Chae HS
• An CY
• Pel P
• Kim YM
• Chin YW

• KMID: 2524102
• DOI: http://doi.org/10.20307/nps.2021.27.4.228

Abstract

Bioactivity-guided fractionation by preliminary screening using interleukin-1β production in lipopolysaccharides (LPS)-induced J774A.1 cell line led to the isolation of fourteen structures including chromone, isocoumarins, flavanoids, and triterpenes from the aerial part of Agrimonia pilosa Ledeb. All structures were determined by measuring their spectroscopic data and comparing their spectroscopic data with the literatures. All the isolates were tested for their inhibitory activities against interleukin-1β production in LPSinduced J774A.1 cell. Of the tested compounds, (S)-(+)-5,7-dihydroxy-2-(1-methylpropyl)chromone (1), agrimonolide-6-O-β-D-glucopyranoside (5), agrimonolide-6-O-α-L-arabinofuranosyl-(1→6)-β-D-glucopyranoside (6), and catechin (10) were found to be active. Furthermore, compound 1 suppressed the protein expressions of NLRP3 and NLRC4 in murine macrophage.

Keyword

Agrimonia pilosa; interleukin-1β; chromone; isocoumarin; inflammation; NLRP3; NLRC4; inflammasome